一种深海微生物脂肪酶在非水介质中催化合成乙酸肉桂酯的应用研究  

Synthesis of cinnamyl acetate by lipase- catalyzed trans- esterification

作　　者：王召贺 张云[2,3] 孙爱君[2,3] 杨再昌[1] 胡云峰[2,3] 

机构地区：[1]贵州大学酿酒与食品工程学院,贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]中国科学院南海海洋研究所,热带海洋生物资源与生态重点实验室,广东广州510301 [3]中国科学院南海海洋研究所,广东省海洋药物重点实验室,广东广州510301

出　　处：《食品工业科技》2016年第9期202-207,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金项目(21302199);中国科学院战略性先导科技专项(XDA11030404);中国科学院重点部署项目(KGZD-EW-606);广州市科技计划项目(201510010012)

摘　　要：从一株深海来源微生物Streptomyces sp.SCSIO 13580的基因组中克隆了1个序列新颖的脂肪酶编码基因,含有1005个碱基,编码334个氨基酸,与最相近的蛋白有51%相似。在大肠杆菌中异源表达了这个脂肪酶,发现其具有在非水介质中催化合成乙酸肉桂酯的活性。在单因素实验的基础上,考察了酰基供体、溶剂、肉桂醇/乙酸乙烯酯摩尔浓度之比、温度等因素对该酶合成乙酸肉桂酯的活性的影响,得到的最佳反应条件是:以乙酸乙烯酯为酰基供体,以正己烷为溶剂,肉桂醇/乙酸乙烯酯的摩尔浓度之比为1∶6,反应温度为40℃,优化后的转化率达到48.3%,酶粉的酶活力为5.59 U/g。One novel gene encoding a lipase L- 1 was cloned from the genome of Streptomyces sp.SCSIO 13580 identified from the deep sea of the South China Sea.L- 1 contains 1005 bp and encodes a putative lipase that harbors 334 amino acids and exhibits the highest similarity of 51% with other lipases.The lipase was heterologously expressed in E.coli.The lipase could catalyze the trans- esterification reaction of vinyl acetate and cinnamyl alcohol in non- aqueous medium.The effects including cinnamyl alcohol / vinyl acetate ratio（ mol / mol）,temperature,solvents and aclyl donors,on the catalytic activities were further investigated.The optimum enzymatic working conditions were obtained as follows： vinyl acetate as the acyl donor,n- hexane as the solvent,ratio of cinnamyl alcohol / vinyl acetate 1 ∶ 6,reaction temperature of 40 ℃,the maximum conversion rate could reach 48.3%,the activity of enzyme powder was 5.59 U / g.

关 键 词：脂肪酶 转酯反应 乙酸肉桂酯 肉桂醇 乙酸乙烯酯 

分 类 号：TS264.3[轻工技术与工程—发酵工程]