超声波辅助提取中国木茎皮多糖的工艺优化及抗氧化活性研究  

Optimization of ultrasonic- assisted extraction of polysaccharides from Aralia chinensis L.stem-bark and its antioxidant activities evaluation

作　　者：靳洁[1] 王一峰[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《食品工业科技》2016年第9期218-223,234,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31460105);兰州市科技局项目(2013-4-89)

摘　　要：以中国楤木(Aralia chinensis L.)茎皮为原料,研究了超声波辅助提取中国楤木茎皮多糖的最优工艺条件及体外抗氧化活性。根据Box-Behnken设计原理,在单因素实验基础上,选取液料比、提取温度、超声功率和提取时间四个因素进行四因素三水平的响应面实验,结果表明超声波辅助提取多糖的最优条件为:液料比33 m L/g、提取温度71℃、超声功率124 W、提取时间93 min,该条件下多糖提取率预测值为12.897%,验证值为12.871%±0.11%。体外抗氧化活性研究结果显示:在实验范围内,随着浓度的升高,中国楤木茎皮多糖对DPPH自由基、羟基自由基、超氧阴离子的清除能力逐渐增强,还原能力也逐渐增强。表明该响应面实验产生的回归模型用于优化中国楤木茎皮多糖的超声波提取工艺是可行的;中国楤木茎皮多糖具有一定的体外抗氧化能力,可以考虑将其开发成为一种天然抗氧化剂在医学或功能食品领域中使用。Using Aralia chinensis L.stem- bark as materials,optimal process conditions of ultrasonic- assisted extraction of Aralia chinensis L.stem- bark polysaccharides（ ACSP） and its antioxidant activities were studied.According to the Box- Behnken design principle,based on the single factor experiment,four- factor（ ratio of water to material,extraction temperature,ultrasonic power and extraction time） three- level response surface methodology（ RSM） was conducted.The optimal extraction conditions of ACSP were determined as follows： ratio of water to material was 33 m L / g,extraction temperature was 71 ℃,ultrasonic power was 124 W,extraction time was93 min.Under optimal conditions,predicted value of polysaccharides yield was 12.897%,verified value was 12.871± 0.11%.The results of antioxidant activities of ACSP in vitro showed that the scavenging activity of DPPH,hydroxyl radical,superoxide radical and reducing power increased with the increase of polysaccharides concentration in the experimental range.Conclusion： the regression model generated from RSM was feasible to optimize the ultrasonicassisted extraction process of ACSP.ACSP has good antioxidant activity,which may be explored as a natural antioxidant in medicine or functional foods areas.

关 键 词：中国楤木茎皮多糖 超声波辅助提取 响应面法 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]