亚麻籽中酚酸化合物的鉴定与含量测定  

The Identification and Content Determination of Phenolic Acid Compounds in Flax Seeds

作　　者：李群[1] 胡晓军[1] 许光映[1] 高忠东[1] 

机构地区：[1]山西省农业科学院农产品加工研究所,山西太原030031

出　　处：《核农学报》2016年第3期493-501,共9页Journal of Nuclear Agricultural Sciences

基　　金：山西省科技攻关项目(20130311032-1)

摘　　要：为了揭示亚麻籽中酚酸类化合物的组成、形态及分布情况,采用反相高效液相色谱法-二极管阵列技术,对亚麻籽粉乙醇提取物的碱解、酶解产物进行鉴定与分析。结果表明,开环异落叶松树脂素酚二葡萄糖甙(SDG)、阿魏酸和对香豆酸以稳定的结合态存在于亚麻种子中;不同水解方法对酚酸化合物的释放量具有显著影响,高温(120℃)碱解条件下SDG的释出量最大;不同品种间各组成成分基本相同,但含量差异显著,其中SDG的质量分数为8~16.5mg·g^(-1),对香豆酸、阿魏酸的质量分数为0.3~1.35mg·g^(-1),阿魏酸与对香豆酸含量之间存在显著的相关性(R=0.89*)。本研究为亚麻籽酚酸资源开发过程中的原料选择、工艺确定、质量控制等环节提供了技术支持。In order to reveal the composition,distribution and morphology of phenolic compounds in flaxseed,RP-HPLC- diode array（ RP- HPLC- DAD） technology was used to identify and analyze the product which has been alkaline hydrolyzed and digested from flaxseed ethanol extract. The results show that the open- loop two different larch resin,phenol prime glucoside（ secoisolariciresinol diglucoside- SDG）,ferulic acid and p- coumaric acid should be bound to a stable presence in flax seeds. Different hydrolysis methods have significantly effects on the release of phenolic acid compounds,the most SDG released quantity was obtained under the condition of alkaline hydrolysis at 120℃temperature. There is the same composition and significant different quantity in different varieties. Mass fraction of SDG is between 8 mg·g^-1and 16. 5mg·g^-1. That of p- coumaric acid and ferulic acid content is between 0. 3 mg·g^-1and 1. 35mg·g^-1. There is significant correlation（ R = 0. 89＊） between p- coumaric acid and ferulic acid content.And the results could provide technical proposal on raw material selection,process formulation and quality control during flaxseed acid resource development.

关 键 词：亚麻籽 反相高效液相色谱法 SDG 阿魏酸 对香豆酸 

分 类 号：S563.2[农业科学—作物学]