响应面法优化带鱼下脚料酶解物螯合锌的制备工艺  

Optimization of Chelation of Hairtail Off-cuts Hydrolysates With Zinc by Response Surface Methodology

作　　者：董亚飞[1] 徐彤砚 杨文鸽[1] 徐大伦[1] 童丽娟[1] 

机构地区：[1]宁波大学海洋学院/浙江海洋高效健康养殖协同创新中心,浙江宁波315211

出　　处：《核农学报》2016年第3期541-547,共7页Journal of Nuclear Agricultural Sciences

基　　金：海洋公益性行业科研专项(201305013);宁波大学学科项目(XKL14D2086)

摘　　要：为优化带鱼下脚料酶解物和锌离子的螯合工艺条件,以带鱼加工下脚料为原料,在单因素试验的基础上,采用Box-Behnken中心组合设计和响应面分析法,探讨酶解物与硫酸锌的质量比、酶解物浓度、p H、反应温度、反应时间等因素对酶解物锌螯合反应的影响。结果表明,带鱼下脚料酶解物螯合锌的最适条件为:质量比4.5∶1,p H值6.0,酶解物浓度4%,反应温度50℃,反应时间40 min,在此条件下螯合率达到84.16%,与模型预测值相符。该螯合工艺反应条件温和,锌螯合率高。本研究为锌螯合物的制备以及带鱼下脚料的高值化利用提供了理论依据。In order to optimize the chelating conditions of hydrolysate with zinc,the hairtail off-cuts was hydrolyzed and used for chelating zinc chelate. Based on the single-factor experiments,the Box-Behnken central composite design and response surface method were adopted to investigate the effect of weight ratio,pH,hydrolysate concentration,chelate temperature and time on the chelation rate. The optimal chelate conditions were determined as follows： weight ratio4. 5∶ 1,pH 6,hydrolysate concentration 4%,chelate temperature 50℃,chelate time 40 min,respectively. Under the optimized conditions,the chelating rate of hydrolysate with zinc could reach 84. 16%,which basically accorded with the model predicted value. The above mentioned chelate process had the advantages of mild reaction condition and high zinc chelating rate,which provided theoretical foundation for the development of hydrolysate chelating zinc and high-value utilization of hairtail off-cuts.

关 键 词：带鱼下脚料 酶解物 锌 螯合 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]