一株红色素产生菌的鉴定及发酵条件优化  

Optimization of red pigment by P.agaridevorans XF-105 using response surface methodology

作　　者：左勇[1] 傅彬[1] 杨小龙[1] 叶碧霞[1] 张晶[1] 

机构地区：[1]四川理工学院生物工程学院,自贡643000

出　　处：《中国食品添加剂》2016年第4期81-89,共9页China Food Additives

基　　金：四川省科技计划项目(2010NZ0063);四川理工学院研究生创新基金项目(y2014029)

摘　　要：将一株从四川自贡砂质土壤中分离得到的产红色素菌株XF105采用生理生化及分子生物学鉴定,根据菌株的16SrDNA序列分析结果及生理生化特性,该菌株XF105归属为P.agaridevorans属。以此菌株为出发菌株,分别从发酵温度、p H、装液量、接种量4个单因素研究发酵条件,在单因素实验基础上采用响应曲面设计实验,对Paenibacillus agaridevorans XF-105产红色素的发酵条件进行优化。优化结果表明,Paenibacillus agaridevorans XF-105产红色素最优发酵条件为温度27℃,p H6.15,装液量为22.4%(v/v),接种量为9.78%(v/v),在此条件下红色素的吸光度为0.415,回归模型的预测值为0.3996,发酵效果最佳。The strain of producing red pigment,isolated from soil where from Zigong,was identified with the method of physiology and biochemistry and molecular biology. The strain XF105 was identified as P. agaridevoransaccording to results of morphological and cultural characteristics and the 16 SrDNA gene sequence. On the basis of single-tests,culture temperature,p H,volume broth,inoculation effect of production of red pigment was optimized using response surface methodlogy. The results as follows：culture temperature of 27℃,intial p H of 6.15,volume broth of 22.4 %（v/v）,and inoculation amount of 9.78 %（v/v）. The red pigment of absorbance was 0.415,and the regression model of prediction was 0.3996 under the optimal conditions.

关 键 词：菌种鉴定 红色素 发酵条件优化 响应面法 

分 类 号：TS202.3[轻工技术与工程—食品科学]