脱盐乳清粉代谢产物的抗氧化和抗肿瘤活性初步研究  

A preliminary study on antioxidant activity and antitumor activity of desalted whey powder metabolites

作　　者：侯惠玲[1] 关宏[1] 郭皓[1] 陈雪[1] 刘振艳[1] 刘宇超[1] 藏传刚[1] 杨文钦[1] 

机构地区：[1]齐齐哈尔医学院,齐齐哈尔161006

出　　处：《中国食品添加剂》2016年第4期104-109,共6页China Food Additives

基　　金：齐齐哈尔市科学技术局计划项目(SFGG-201335)

摘　　要：比较研究以脱盐乳清粉为主要原料,采用乳杆菌A-2进行发酵制备的代谢产物(LF),并初步探讨了LF抗氧化和抗肿瘤活性的功能。通过对发酵液的p H、总糖和Ca^(2+)含量的测定,筛选出最佳菌悬液浓度为1OD/mL;用ABTS法和ORAC法来测定和评价乳杆菌A-2发酵产物的抗氧化作用。在反应体系中,脱盐乳清粉和LF的ABTS清除率的IC50分别为34.29mg/mL和19.74mg/mL,LF的ABTS清除率的能力优于脱盐乳清粉;其相对ORAC值分别为75.46μmol Trolox/g和114.78μmol Trolox/g,LF清除AAPH的能力为脱盐乳清粉的1.5倍,结果显示LF具有一定抗氧化活性。采用MTT法检测LF对人舌癌细胞系Cal-27细胞增殖的影响,LF的IC50为19.1 mg/mL,且浓度在4~32 mg/mL的范围内,对人舌癌细胞系Cal-27细胞的抑制率呈剂量依赖性,与脱盐乳清粉组比较,差异具有显著性。In this study,the desalted whey powder was used as the principal raw material,using Lactobacillus A-2 for fermentation,to produce antioxidant activity and antitumor activity metabolite（LF）. By measuring PH value of fermentation bacteria,total sugar and Ca（2＋）concentration,the best concentration of the bacterial suspension is determined as 1OD /mL;the antioxidant activity of Lactobacillus A-2 whey fermentation product was evaluated by ABTS and ORAC. In the reaction system,the ABTS free radical scavenging activity IC50 of desalted whey powder and LF were 34.29mg/mL and 19.74mg/mL respectively,the ABTS free radical scavenging activity of LF is better than that of desalted whey powder;the ORAC assay of desalted whey powder and LF were 75.4 and 114.78μmol Trolox/g respectively. The results showed that LF had prominent antioxidant activity. Using MTT method to measure the influence of desalted whey powder and LF on Human tongue squamous carcinoma cells Cal-27 cell proliferation,the concentration of LF between 432 mg/mL had inhibition effect of Human tongue squamous carcinoma cells Cal-27 function on a dose-dependent manner,and the difference was significant compare with the desalted whey powder.

关 键 词：脱盐乳清粉 发酵 抗氧化性 抗肿瘤活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]