炭疽病病原菌侵染对茶树叶片生理特性的影响  

Changes in physiological properties of tea leaf infected by Colletotrichum kahawae

作　　者：赖建东[1] 魏日凤[1] 连玲丽[2] 刘伟[3,4] 叶乃兴[1,3] 杨江帆[3,5,6] 陈丹[1] 杨国一[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002 [3]福建农林大学茶叶科技与经济研究所,福建福州350002 [4]宁德师范学院生物系,福建宁德352000 [5]武夷学院,福建武夷山354300 [6]中国乌龙茶产业协同创新中心,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2016年第2期162-168,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省"2011协同创新中心"项目(2015);宁德师范学院引进人才项目(2014Y001)

摘　　要：本试验筛选出一株强致病力茶树刺盘孢菌株ZRG,并测定其侵染后对茶树叶片相对电导率,丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等抗氧化酶活性及其基因表达量的影响,探究茶树刺盘孢对茶树叶片生理特性的影响.结果表明:ZRG侵染48 h内,茶树叶片相对电导率明显增高;MDA先是快速积累,含量上升,随后下降;POD、SOD和CAT活性都出现不同程度的上升,随后下降.qRT-PCR验证结果显示,POD、SOD和CAT相关基因的表达先上调后下降,三者相互协调配合,起到清除过剩自由基的作用.To investigate pathogenicity of Colletotrichum sp. on tea leaves,a virulent strain ZRG of Colletotrichum kahawaewas isolated to infect 7 types of tea leaves that were commonly grown in Fujian. Physiological parameters,including relative conductivity,malondialdehyde（ MDA） concentration and three types of antioxidase,i. e. superoxide dismutase（ SOD）,peroxidase（ POD） and catalase（ CAT）,were monitored at 0,24,48,72 hours after infection. Subsequently,qRT-PCR was applied to express genes that were relevant to SOD,POD and CAT. Results showed that relative conductivities of infected leaves were significantly enhanced within 48 h after infection. Concentrations of MDA,SOD,POD and CAT all demonstrated similar pattern,which peaked around 24 th or48th hour after inoculation and decreased afterwards. Correspondingly,qRT-PCR on SOD,POD and CAT genes detected upregulated expressions of three antioxidases in infected leaves.

关 键 词：茶树 炭疽病病原菌 生理特性 QRT-PCR 

分 类 号：S571.1[农业科学—茶叶生产加工] S435.621.22[农业科学—作物学]