高温大曲中高产糖化酶菌株的筛选鉴定及固态发酵条件优化  

Filtering and Identifying of High-yield Glucoamylase Strains from High Temperature Daqu and Optimization of Solid State Fermentation Conditions

作　　者：鲁珍[1] 刘家扬[1] 谌馥佳[1] 李恩中[1] 杨锟[1] 张建设 郜胜勤 

机构地区：[1]黄淮学院生物工程系,河南驻马店463000 [2]河南豫坡酒业有限责任公司,河南西平463900

出　　处：《湖南农业科学》2016年第4期1-4,共4页Hunan Agricultural Sciences

基　　金：国家青年自然科学基金项目(51503074);2015年河南省重点科技攻关项目(152102210025)

摘　　要：对高温大曲中高产糖化酶菌株进行分离和鉴定,并对筛选得到的菌株进行发酵条件的优化。结果表明:试验前期获得的8株产酱香菌株中GX06菌株产糖化酶能力最强,同等条件下其糖化酶活力为706.13 U/g;通过形态观察及16S r DNA序列同源性分析,初步鉴定该菌株为Bacillus cereus;通过单因素和正交试验确定该菌株的最佳产酶条件为:以尿素为最佳氮源,以麸皮为最佳碳源,初始p H值6.0,接种量9%,在50℃下发酵70 h后,其产物糖化酶活性可达917.03 U/g。Isolate and identify high-yield glucoamylase strains from high temperature Daqu and optimize the fermentation conditions of filtered strains. The results showed that, the 8 Jiangxiang-producing strains obtained from early stage of test, the GX06 strain has the strongest ability of producing glucoamylase, activity of glucoamylase is 706.13 U/g under the same conditions; by morphological observation and r DNA 16 S sequence homology analysis, the strain was identified as Bacillus cereus; the best enzyme production conditions of the strain were determined by single factor and orthogonal test： using urea as the best nitrogen source, using wheat bran as the best carbon source, initial p H value of 6.0, inoculation amount of 9%, after 70 h fermentation under 50 ℃, the glucoamylase activity of its production is up to 917.03 U/g.

关 键 词：高温大曲 糖化酶 鉴定 优化 

分 类 号：Q93-3[生物学—微生物学]