姜黄精油的抗氧化活性研究  

Study on antioxidant activity of turmeric essential oil

作　　者：魏娟[1] 苏海兰[1] 张溪桐[1] 毕阳[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《食品工业科技》2016年第10期141-144,149,共5页Science and Technology of Food Industry

基　　金：甘肃省青年科技基金计划(1208RJYA095);国家自然科学基金(31360382)

摘　　要：姜黄为姜科姜黄属的多年生草本植物,是一种药食两用材料。采用水蒸汽蒸馏法提取了姜黄精油,研究了其对ABTS+自由基、羟自由基的清除率及其抑制亚油酸脂质过氧化的能力,通过MTS法研究了其对HepG2细胞的细胞毒作用,采用流式细胞技术评估了其在细胞水平的抗氧化能力。结果表明:姜黄精油对ABTS+自由基具有良好的清除效果,但其清除能力低于V_E;对羟自由基的清除效果非常明显,与V_E的清除能力相当;并且能有效抑制亚油酸脂质过氧化。姜黄精油在0.02%(v/v)浓度范围内没有明显的细胞毒作用,在此浓度范围内能有效清除HepG2细胞内的活性氧。由此表明,姜黄精油具有开发为安全天然的食品抗氧化剂的潜力。Turmeric（ Curcuma longa L.） is a plant belonging to the family of Zingiberaceae,it has been commonly used in Chinese traditional medicine and food coloring. In this study,the turmeric essential oil was extracted by steam distillation,and its antioxidant capacity was evaluated based on ABTS radical and hydroxyl free radical（ ·OH） scavenging activities,inhibition of lipid peroxidation in linoleie acid. Its cytotoxic effect on Hep G2 cells was evaluated by mitochondrial- respiration- dependent 3-（ 4,5- dimethylthiazol- 2- yl）- 2,5- diphenyltetrazolium（ MTS） assay,the antioxidant effect in cell levels was investigated by flow cytometry. The results indicated that turmeric essential oil exhibited good scavenging activities against ABTS free radicals,but the scavenging rate was lower than that of V_E.The essential oil could significantly scavenge ·OH radicals,the scavenging rate was similar with that of V_E. And it could inhibit lipid peroxidation in linoleie acid. Within the concentration of 0.02%（ v / v）,the essential oil had no obvious cytotoxicity on Hep G2 cells,and could scavenge the reactive oxygen in them. It is suggested that turmeric essential oil could be developed as a food addictive of natural antioxidants.

关 键 词：姜黄 精油 抗氧化 

分 类 号：TS201.3[轻工技术与工程—食品科学]