一株优良果脯加工废糖液酒精发酵酵母菌生长条件的研究  

Research on the Growth Conditions of a Quality Yeast Strai for Alcoholic Fermentation of Waste Syrup from Apple Preserves Production

作　　者：成少宁 许先猛 马欣 张增帅 郭俊花 

机构地区：[1]运城职业技术学院有机食品工程系,山西运城044000

出　　处：《漯河职业技术学院学报》2016年第2期12-15,共4页Journal of Luohe Vocational Technical College

基　　金：山西省高等学校科技创新项目(项目编号:20141123)

摘　　要：本研究采用浊度法,利用分光光度计测定菌液在560 nm波长的OD值,确定细菌浓度。通过测定酵母D7的生长曲线确定其生长周期,进而确定其培养时间;采用单因素试验确定出酵母菌生长的温度、转速、p H值的合适水平,正交试验L9(34)对生长条件进行优化,研究不同的温度、转速、p H值对优良废糖液酒精发酵酵母D7生长的影响。结果确定为16小时为酵母菌后续试验的培养时间;酵母菌的正交试验温度水平为30℃、32℃、34℃,转速水平为160 r/min、180 r/min、200 r/min,p H值水平为3、4、5;正交试验优化的最适生长条件为温度30℃、转速160 r/min、p H值为3。The vaccine OD value was measured by nephelometry at wave length of 560 nm to determine its cell concentration. The growth cycle and culture time was measured by growth curve of D7 strain. The suitable level of temperature,rotational speed and p H value of yeast growth was determined by single factor experiment. Orthogonal experiments L_9（ 3~4） to optimize the growth conditions,the effects of p H value,temperature and rotational speed on the growth of D7 strain was studied. The results shows that the incubation time was 16 hours of follow-up experiments,level of orthogonal experiments temperature is 30℃,32℃,34℃,the speed level of 160 r / min,180 r / min,200 r /min,p H value level 3,4,5; Its optimum growth conditions were determined by orthogonal experiments as follows：temperature at 30℃,rotation speed 160 r / min,p H value of 3.

关 键 词：废糖液 酵母菌 生长条件 

分 类 号：TS261.11[轻工技术与工程—发酵工程]