响应面法优化超声辅助芸豆蛋白糖基化反应  

Optimization of condition for ultrasonic-assisted grafting of red kidney bean protein by response surface method

作　　者：李玉琼[1] 王长远[1] 全越[1] 冯玉超[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2016年第5期538-543,547,共7页Chinese Journal of Biologicals

基　　金：黑龙江省科技重大项目(GA15B301)

摘　　要：目的响应面法优化超声辅助芸豆蛋白糖基化反应。方法利用超声波辅助机制,对芸豆蛋白进行糖基化改性。通过单因素试验,在确定反应温度、超声功率、超声时间、p H、芸豆蛋白与氨基葡萄糖质量比、芸豆蛋白浓度对反应接枝度影响的基础上,通过Design-Expert 8.0.6.1软件做响应面分析。结果最终确定最佳反应条件为:反应温度70℃,超声功率270 W,超声时间40 min,p H 9.75,芸豆蛋白与氨基葡萄糖质量比1∶2,芸豆蛋白浓度9 mg/ml;在此条件下进行验证试验,接枝度为39.576 1%,与模型所得理论接枝度(39.631 0%)相差0.054 9%。结论成功优化了超声辅助芸豆蛋白糖基化反应,确定了最佳工艺,为改善芸豆蛋白的功能性以拓宽其应用范围提供了参考。Objective To optimize the condition for ultrasonic-assisted grafting of red kidney bean protein by response surface method.Methods The grafting of red kidney bean protein was performed by ultrasonic-assisted mechanism.The effect of reaction temperature,ultrasonic power,time,p H value,mass ratio of red kidney bean protein to glucosamine and red kidney bean protein concentration on degree of grafting（DG）were analyzed by single-factor experiment,based on which response surface analysis was performed by Design-Expert 8.0.6.1 software.Results The optimal reaction temperature,ultrasonic power,ultrasonic time,p H value,the mass ratio of red kidney bean protein to glucosamine and red kidney bean protein concentration were 70 ℃,270 W,40 min,9.75,1 ∶ 2 and 9 mg / ml respectively.Under the condition,the actual DG was 39.576 1%,of which the difference from the theoretical value of 39.631 0% was 0.074%.Conclusion The condition for ultrasonic-assisted grafting of red kidney bean protein was successfully optimized,which provided a reference for the modification of function and extending the utilization of red kidney bean protein.

关 键 词：氨基葡萄糖 超声波辅助 芸豆蛋白 接枝度 响应面法 

分 类 号：Q518.4[生物学—生物化学]