高产脂肪酶菌株的筛选及产酶条件研究  

Study on screening of high lipase-producing strain and conditions for enzyme production

作　　者：赵兴秀[1] 赵长青[1] 何义国[1] 邹伟[1] 张静[1] 

机构地区：[1]四川理工学院,四川自贡643000

出　　处：《食品与机械》2016年第3期16-19,76,共5页Food and Machinery

基　　金：酿酒生物技术及应用四川省重点实验室项目(编号:NJ2012-16);四川理工学院科研项目(编号:2012PY08;2012RC10);四川省教育厅项目(编号:14ZA0206);泸州老窖科研奖学金项目(编号:13ljzk05);绿色催化四川省重点实验室(编号:LYJ1304)

摘　　要：从油坊土壤、饭店、食堂油烟机排气孔等8个取样点获得16份样品,通过溴甲酚紫平板初筛和摇瓶发酵复筛选出9株产脂肪酶量高的菌株,其中一菌株A7的产量最高,其酶活为12.9U/mL,以菌株A7为出发菌株再进行发酵培养基的研究。经过单因素和正交试验,确定脂肪酶高产的最佳培养基为:大豆油5g/L、蛋白胨20g/L、MgSO_47H_2O 0.4g/L、初始pH 6.0,培养温度30℃,摇床转速200r/min,优化后产酶量达到19.8U/mL。16 samples were obtained from 8 sample points such as the soil of oil mill, restaurant, lampblack absorber. 9 strains were got ten by primary screening with bromocresol purple plate and seconda ry screening with shaking flask fermentation, and a strain, which producing lipase with 12.9 U/mL was determined original strain. By single factor and orthogonal experiment the optimum condition for high lipase producing of the strain A7 was obtained, which was soybean oil 5 g/L, peptone 20 g/L, MgSO4 ·7H2O 0.4 g/L, pH 6.0, culture temperature 30 ℃ , rotation speed 200 r/min. The enzyme activity of strain A7 was 19.8 U/mL under the optimtnn condition.

关 键 词：脂肪酶 筛选 培养基 生物柴油 

分 类 号：TE667[石油与天然气工程—油气加工工程] TQ925[轻工技术与工程—发酵工程]