高产角蛋白酶耐盐菌Bacillus sp.K-18的筛选及发酵特性研究  

Screening of a Halo-tolerant Bacillus sp. K-18 with High Keratinase Production and its Characterization of Fermentation

作　　者：侯燕燕[1,2] 汤城岸 鲁凤娟[1,2] 张鹤铭 龙秀锋 张帅[1,2] 田永强[1,2] 

机构地区：[1]四川大学皮革化学与工程教育部重点实验室,四川成都610065 [2]四川大学制革清洁技术国家工程实验室,四川成都610065

出　　处：《皮革科学与工程》2016年第3期25-29,共5页Leather Science and Engineering

基　　金：中央高校基本科研业务费专项资金资助(SCU2015D008);四川应用基础研究计划项目(2014JY0199)

摘　　要：通过酪蛋白初筛、羊毛降解复筛,选出一株高效产角蛋白酶耐盐菌K-18,结合菌株的形态学观察和生理生化特性,鉴定该菌为Bacillus sp.。对其进行产酶发酵条件优化,得出最佳培养基配方为:葡萄糖5.9 g/L,胰蛋白胨10.1 g/L,最佳培养条件为:培养温度39℃,初始培养基pH值8,培养时间60 h,在此条件下,菌株发酵液角蛋白酶活力达到289.9 U/m L。该耐盐角蛋白酶在盐腌皮脱毛工业方面具有一定的应用前景。A halo-tolerant strain K-18 with high keratinase production was obtained by casein plate firstly and the degradation of wool secondly, the strain K-18 was identified as Bacillus sp. by morphological observation and physiological-biochemical properties. Fermentation conditions of strain K-18 were optimized, the results showed that using appropriate carbon source （glucose 5.9 g/L） or nitrogen source （tryptone 10.1 g/L）, the optimum temperature,initial pH values and incubation time of strain K-18 was 39 ℃, 8, and 60 h, 289.9 U/mL keratinase activity was obtained in this condition. The halo-tolerant strain K-18 has great potential in unhairing of salt cured hide.

关 键 词：耐盐菌 角蛋白酶 发酵特性 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]