高产中性蛋白酶菌株的筛选及其产酶条件的研究  

Screening and Isolation of High Neutral Protease Yielding Strains and Study on the Best Fermentation Performance

作　　者：鲁珍[1] 谌馥佳[1] 李恩中[1] 刘家扬[1] 卢自周 张建设 郜胜勤 

机构地区：[1]黄淮学院生物工程系,河南驻马店463000 [2]河南豫坡酒业有限责任公司,河南西平463900

出　　处：《黑龙江农业科学》2016年第5期110-114,共5页Heilongjiang Agricultural Sciences

基　　金：国家青年自然科学基金资助项目(51503074);2015年河南省重点科技攻关资助项目(152102210025)

摘　　要：为从高温大曲中筛选出高产中性蛋白酶菌株,利用生理生化实验和16SrDNA序列同源性鉴定和分析产酶条件。结查表明:从已获得的8株产酱香菌株筛选得到高产中性蛋白酶菌株为GX03(706.13U·g-1),并鉴定该菌株为解淀粉芽孢杆菌。通过单因素和正交试验得到最适产酶条件:含水量为35%,接种量为11%,氮源为硝酸钠,初始pH 5.5,培养时间为6d,经优化后该菌产酶性能大幅度提高,可达567.30U·g-1。In order to extract the strains with high yield neutral protease from high temperature Daqu,enzyme production conditions were studied and authenticated.8Jiangxiang bacterial strains were isolated from the high temperature Daqu before,and the strain GX03 with high-yield of neutral protease was isolated from the 8Jiangxiang bacterial strains.It was identified as Bacillus amyloliquefaciens through the physiological and biochemical characteristics and molecular identification.The optimal condition for the fermentation were as follows：moisture content 35%,the strain inoculation amount 11%,NaNO3 as nitrogen source,the initial pH 5.5,fermentation time for 6days.Under this conditon,the neutral protease was 567.30U·g^-1.

关 键 词：微生物 酱香菌 中性蛋白酶 酶活 

分 类 号：TS261.1[轻工技术与工程—发酵工程] TQ925[轻工技术与工程—食品科学与工程]