液相质谱法测定露酒中的甜蜜素含量  

The Method of Using a Triple Quadrupole Mass Spectrometer for the Determination of Sodium Cyclamate in Lu Jiu

作　　者：许和强 刘嘉飞 王立亚 

机构地区：[1]广东省食品检验所,广东广州510410

出　　处：《酿酒》2016年第3期102-103,共2页Liquor Making

摘　　要：建立了一种使用液相质谱仪测定露酒中的甜蜜素的方法。使用UHPLC二元高压梯度系统利用C18色谱柱在5min内实现快速分离。实验结果表明:线性范围5μg/L^500μg/L,相关系数为0.9995;甜蜜素标样的仪器检出限为1μg/L,仪器定量限为3μg/L;5μg/L、50μg/L和500μg/L三个浓度标样6次连续进样的保留时间和峰面积相对标准偏差分别在0.12%~0.16%和0.91%~2.34%之间;50μg/L露酒样品加标回收率为98.6%。This paper established a method of using a triple quadrupole mass spectrometer for the determination of Sodium cyclamate in Lu Jiu. Using UHPLC binary high pressure gradient system and C18 chromatographic column achieved a rapid separation within 5minutes.The experimental results showed that： the linear range 5μg/L^500μg/L, the correlation coefficient is 0.9995; Sodium cyclamate prototype instrument detection limit of 1μg/L, instrument quantitative limit of 3μg/L; the relative standard deviation of 5μg/L, 50μg/L and 500μg/L of three concentration sample＇s 6 consecutive sample retention time and peak area respectively between 0.1%~ 0.16% and 0.91%~2.34%; 50μg/L Lu Jiu sample standard addition recovery rate was 98.6%.

关 键 词：甜蜜素 UHPLC 露酒 

分 类 号：TS262.7[轻工技术与工程—发酵工程] TS263.3[轻工技术与工程—食品科学与工程]