乳制品中多种常用人工合成色素的同时测定  

Simultaneous determination of various synthetic colorants in dairy products

作　　者：宋戈[1] 亢美娟[1] 苗晶[1] 

机构地区：[1]东北农业大学国家乳制品质量监督检验中心,哈尔滨150086

出　　处：《中国乳品工业》2016年第5期43-47,共5页China Dairy Industry

摘　　要：建立了高效液相色谱法同时测定乳及乳制品中11种常用人工合成色素的方法。通过加入适量沉淀剂除去样品中蛋白质后,经氨水甲醇溶液提取,混合型聚酰胺固相萃取柱净化浓缩后,采用C18色谱柱,以乙腈-0.02 mol/L乙酸铵溶液为流动相梯度洗脱,用二极管阵列检测器多波长检测。回收率在92.0%~103.5%,精密度（RSD）为1.89%~3.96%,检出限在2.0~15.0 ug/kg。该方法可用于乳及乳制品中人工合成色素的日常检测。A method for Simultaneous determination of 11 kinds synthetic colorants in milk and dairy products by high-performance liquid chromatography（HPLC） was established. Protein in milk and dairy products were mostly eliminated by the precipitators, extracted with ammonia-methanol, purified and enriched by WAX extraction cartridges, separated on C18 column with a mixed solution of acetonitrile-0.02mol/L ammonium acetate as mobile phase by gradient elution, and detected with a photo-diode array detector. The recovery were 92.0%~103.5%,the relative standard deviations（RSD） were 1.89%~3.96%, the minimum detectable values were 2.0~15.0 ug/kg. This method can be used for the routine analysis of synthetic colorants in milk and dairy products.

关 键 词：人工合成色素 乳制品 高效液相色谱 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]