QuEChERS-超高效液相色谱-串联质谱法快速测定饮料中13种饱和内酯类合成香料  

Rapid Determination of 13 Synthetic Saturated Lactones Added as Flavors in Beverage by QuEChERS Method Coupled with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：王玉健[1] 纪少凡 陈文慧[1] 黄惠玲[1] 董存柱[2] 

机构地区：[1]海南出入境检验检疫局检验检疫技术中心,海南海口570311 [2]海南大学环境与植物保护学院,海南海口570228

出　　处：《食品科学》2016年第12期197-202,共6页Food Science

基　　金：国家质检总局科技计划项目(2013IK153);海南省重点科技计划项目(ZDXM2014083);国家自然科学基金青年科学基金项目(31360447);海南省自然科学基金项目(212018;313105)

摘　　要：建立超高效液相色谱—串联质谱法快速测定饮料中13种饱和内酯类合成香料的方法。采用QuEChERS前处理法,以乙腈提取,N-丙基乙二胺填料吸附剂直接净化,电喷雾离子源、多反应监测正离子模式扫描,外标法定量。13种饱和内酯在0.025-0.50μg/mL范围内质量浓度与峰面积呈良好的线性关系,仪器检出限为0.025μg/mL,方法的检出限为0.10 mg/kg,定量限为0.40 mg/kg,添加量为0.10、0.20、0.40 mg/kg时13种饱和内酯的平均回收率范围分别为88.5%-103%,相对标准偏差为3.8%-11%（n=10）。本方法可用于饮料中饱和内酯类化合物的快速定性、定量检测。A method for determining 13 synthetic saturated lactones added as flavors in beverage by ultra performanceliquid chromatography-tandem mass spectrometry （UPLC-MS/MS） was established. The target compounds wereextracted with acetonitrile and determined by quick easy cheap effective rugged and safe （QuEChERS） pretreatmentcoupled with UPLC-MS/MS （under MRM mode） with an external standard method. The limits of detection （LODs） were0.025 μg/mL for the instrument and 0.10 mg/kg for the method, respectively. The limit of quantitation （LOQ） was0.40 mg/kg for the method. The average recoveries of 13 lactones were between 88.5% and 103% at spiked levels of 0.10,0.20 and 0.40 mg/kg with relative standard derivation （RSDs） of 3.8%–11% （n = 10）. The method was reliable and allowedsimultaneous quantitative determination of saturated lactones in beverage.

关 键 词：饱和内酯类香料 饮料 QUECHERS 超高效液相色谱—串联质谱 

分 类 号：TS262.6[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]