微生物红外鉴定仪在结冷胶高产菌株选育中的应用探索  

Application of FTIR in screening of high-yield gellan gum producing strains

作　　者：汪钊[1] 徐力[1] 魏春[1] 

机构地区：[1]浙江工业大学生物工程学院,浙江杭州310014

出　　处：《发酵科技通讯》2016年第2期65-69,共5页Bulletin of Fermentation Science and Technology

摘　　要：利用利福平等抗生素对结冷胶生产菌株Sphingomonas elodea WZW002进行诱变处理,同时根据傅立叶红外光谱(FTIR)原理,对微生物红外鉴定谱图进行归一化和基线校正的处理,对比特征峰的吸收强度以及朗伯比尔定律分析糖组分,建立了高效筛选结冷胶高产菌株的方法。筛选得到一株结冷胶高产菌株,产量为17.21 g/L,较原始菌株提高5.8%。A gellan gum producing strain Sphingomonas sp.WZW002 was mutated by rifampicin.Based on infrared spectrum（FTIR） identification, and lambert beer law analysis, a high-throughput screening method was established. A mutant strain with high productivity was screened and the yield of gellan gum reached 17.21 g/L, which increased by 5.8% comparing with the wild type strain.

关 键 词：结冷胶 FTIR 筛选 多糖 

分 类 号：Q93[生物学—微生物学]