高产色素红曲菌的筛选鉴定及其液态发酵条件优化  

Screening and Identifying a High Pigment-producing Monacus Strain,and Optimizing Its Liquid Fermentation

作　　者：贾瑞博[1,2] 周文斌[1,2] 陈竟豪 李燕[1,2] 刘凯丽[1,2] 赵慧[1,2] 刘斌[1,2] 吕旭聪[1,2,3] 

机构地区：[1]国家菌草工程技术研究中心,福建福州350002 [2]福建农林大学食品科学学院,福建福州350002 [3]阳光国际集团科技发展有限公司,福建泉州362000

出　　处：《福建农业学报》2016年第4期424-430,共7页Fujian Journal of Agricultural Sciences

基　　金：中国博士后科学基金项目(2015M570549);福建省教育厅科技计划项目A类课题(JA14107);福建省自然科学基金项目(2016J01095)

摘　　要：从福建永春、安溪、古田、南平、三明、龙岩等地收集的红曲米中分离筛选得到了1株高产色素红曲菌L03,利用形态学和分子生物学的方法对其进行鉴定,结果表明为紫色红曲菌Monascus purpureus。同时通过单因素和正交优化试验确定了其最佳的液态发酵条件:以8%的甘油为碳源、1.5%蛋白胨为氮源、硫酸镁0.1%、磷酸二氢钾0.25%、接种量6%、发酵温度32℃、时间9d、摇床转速180r·min-1、250mL容器中装液量100mL,在此液态发酵条件下其总色价平均值为1 578U·mL-1。The Monascus strain,L03,was isolated from the red kojic rice samples collected from Yongchun,Anxi, Gutian,Nanping,Sanming,and Longyan in Fujian for its high red-pigment-producing ability.Based on the morphology and molecular biology of L03,it was identified to be M.purpureus .Subsequently,single factor and orthogonal experiments were conducted to optimize the conditions for the liquid fermentation of the yeast.Color of the fermented suspension reached 1,578 U/mL when the yeast was inoculated 6% by weight into a liquid medium consisting of 8% of glycerol,1.5 % of peptone,0.1% of Mg2 SO4 ,and 0.25% of KH 2 PO4 in a loading volume of 100 mL/250 mL and incubated at 32℃ with constant agitation at the speed of 180 rpm for 9 days.

关 键 词：色素 红曲菌 筛选 鉴定 液态发酵 优化 

分 类 号：S646[农业科学—蔬菜学]