HRM法检测肿瘤细胞PTEN基因突变实验研究  

Study on Detection of PTEN Gene Mutation in Tumor Cells by HRM Method

作　　者：贺华[1] 王腾[1] 彭鑫[1] 苏畅[1] 邵红伟[1] 黄树林[1] 

机构地区：[1]广东药学院生命科学与生物制药学院/广东省生物技术候选药物研究重点实验室,广东广州510006

出　　处：《亚太传统医药》2016年第12期5-7,共3页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金(31100664;31300737;81303292);广东省自然科学基金(10151022401000024;2014A030313586);"重大新药创制"科技重大专项(2009ZX09103-708);东莞市科技计划项目(2011105102027)

摘　　要：目的:探索高分辨率熔解曲线(HRM)法检测肿瘤细胞系PTEN基因突变中的应用。方法:采用HRM法初筛肿瘤细胞系PTEN的突变,测序验证HRM筛选结果,TA克隆测序进一步明确突变情况。结果:HRM初筛出Jurkat细胞PTEN基因存在突变,测序结果与HRM结果一致,TA克隆测序确定Jurkat细胞系PTEN基因为缺失突变。结论:HRM法可准确筛选肿瘤细胞系中PTEN基因突变,适于肿瘤细胞PTEN基因突变筛选。Objective： Explore the application of high resolution melting curve （HRM） method for the detection PTEN gene mutation in different tumor cell lines. Methods. HRM method primaryly screen PTEN mutation of tumor cell line;Sequencing to verify the results of HRM sereening~ TA cloning and sequencing to further clarify the mutation. Results. HRM prove that there are PTEN mutations in the Jurkat cell line, The sequencing results were in agreement with HRM resuLts, TA cloning and sequencing prove PTEN gene deletion mutation in Jurkat cell line. Conclusion： The HRM method can accurately screen PTEN gene mutation in tumor cell lines,which is a suitable for tumor cell PTEN gene mutation screening method.

关 键 词：HRM 突变 PTEN 测序 TA克隆 

分 类 号：R735.3[医药卫生—肿瘤]