不同种驹形氏杆菌合成纤维素最适碳源  

Study on optimal carbon sources of Komagataeibacter spp.for bacterial cellulose synthesis

作　　者：陈华美[1] 李从发[1] 毕继才[1] 章翠[1] 胡淇淞 王艳梅[1] 刘四新[1,2] 

机构地区：[1]海南大学食品学院,海南海口570228 [2]海南大学材料与化工学院,海南海口570228

出　　处：《食品与机械》2016年第4期37-41,共5页Food and Machinery

基　　金：海南省自然科学基金项目资助(编号:314048);海口市热带农产品深加工重点实验室项目(编号:2013-45)

摘　　要：研究不同碳源对驹形氏杆菌的几个不同菌种(Komagataeibacter nataicola、K.hansenii和K.europaeus)在细胞生长、代谢及纤维素(Bacterial cellulose,BC)合成方面的影响,旨在揭示不同纤维素产生菌对碳源的偏好和适应性。结果表明:K.europaeus以果糖为碳源合成BC的产量最大,K.nataicola和K.hansenii利用葡萄糖时产BC的能力最佳。不同菌种合成BC时与其细胞生物量、碳源的消耗量及副产物葡萄糖酸等的积累之间未呈一致的相关性,说明每种菌的碳源消耗与产物合成并非必然与生物量呈正相关,而只需三者间达到合适平衡。该研究可为针对具体原料(碳源)选择合适菌种进行BC生产、提高原料利用效益提供有益借鉴。In order to reveal the preference and adaptability of different cellulose-producing species for the carbon source,the influences of carbon sources on Komagataeibacter spp.（K.nataicola,K.hansenii and K.europaeus）for growth,metabolism and BC synthesis were studied.The main results were as follows：the BC production was the largest by K.europaeus with fructose as the carbon source,while K.nataicolaand K.hansenii with glucose as the carbon source.In addition,the results showed that the ability to synthesize BC by different species was not consistent with the cell biomass,gluconic acid and carbon source consumption,and it indicated that the carbon source consumption and the BC production were not necessary to be relevant to cell growth,but to be properly balanced.Therefore,it is not only very necessary to select the appropriate carbon source for different species,but also can provide a useful reference for the selection of suitable species and improving the utilization efficiency of the specific raw materials for BC production.

关 键 词：驹形氏杆菌 细菌纤维素 碳源适应性 菌种差异 

分 类 号：Q939.9[生物学—微生物学] O636.11[理学—高分子化学]