超高效液相色谱-串联质谱法检测蜂蜜中的甲硝唑含量  

Determination of residues of metronidazole in honey by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：王春民[1] 张秋萍[1] 吴春霞[1] 

机构地区：[1]苏州市疾病预防控制中心,苏州215004

出　　处：《食品安全质量检测学报》2016年第5期1813-1817,共5页Journal of Food Safety and Quality

摘　　要：目的建立超高效液相色谱-串联质谱法（ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS）检测蜂蜜中的甲硝唑含量。方法取混合均匀的蜂蜜样品加入饱和氯化钠溶液混合,再经乙酸乙酯提取,经阳离子交换小柱净化。采用UPLC-MS/MS技术,以多反应监测模式检测,以外标法定量。结果甲硝唑在1~10.0μg/L浓度范围内线性关系良好,相关系数大于0.999,回收率为76%,检出限为0.1μg/kg,相对标准偏差为3.4%（n=6）。结论该方法快速、准确、灵敏,可用于蜂蜜中甲硝唑的检测。Objective To establish a method for the determination of metronidazole in honey samples by ultra performance liquid chromatography-tandem mass spectrometric（UPLC-MS/MS）.Methods Honey samples were mixed with saturated sodium chloride and extracted with ethyl acetate,and then purified by cation exchange column.The metronidazole residues in the extracts were detected using UPLC-MS/MS（ESI＋） with multiple reactions monitoring（MRM）,and quantitated with external standard method.Results Metronidazole had a good linear relationship in the range of 1~10.0 μg/L with the linear correlation coefficient larger than 0.999.The recovery was76%,the limits of detection（LOD） was 0.1 μg/kg,and the relative standard deviation（RSD） was 3.4%（n=6）.Conclusion The proposed method is fast,accurate and sensitive,which is suitable for the determination of metronidazole residues in honey samples.

关 键 词：超高效液相色谱-串联质谱法 蜂蜜 甲硝唑 

分 类 号：O657.63[理学—分析化学] TS207.5[理学—化学]