牡蛎肽对免疫抑制小鼠免疫功能的影响  

Immunomodulatory effect of oyster peptide on immunosuppressed mice

作　　者：许丹[1] 林峰[2] 朱小语 刘文颖[2] 陈晓文[1] 冯金秋[1] 范爱琴[1] 蔡木易[2] 许雅君[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100191 [2]中国食品发酵工业研究院,北京市蛋白功能肽工程技术研究中心,北京100015

出　　处：《北京大学学报（医学版）》2016年第3期392-397,共6页Journal of Peking University:Health Sciences

基　　金：“十二五”国家科技支撑计划项目(2012BAD33B04-02);国家高技术研究发展计划(863计划)项目(2013AA102205-02)资助~~

摘　　要：目的:探讨牡蛎肽对免疫抑制小鼠的免疫调节作用及可能机制。方法:以环磷酰胺(cyclophosphamide,CTX)致免疫低下雌性ICR小鼠为动物模型,观察不同剂量牡蛎肽(0.5 g/kg低剂量组、1.0 g/kg中剂量组、2.0 g/kg高剂量组)干预15 d后对小鼠体重、免疫器官相对重量、免疫器官显微结构、外周血白细胞数目、T淋巴细胞亚群百分比、血清中的细胞因子浓度以及骨髓有核细胞数和DNA含量的影响。结果:腹腔注射CTX模型组小鼠与对照组相比,胸腺和脾脏萎缩,显微结构紊乱,外周血白细胞数目降低(P=0.04),外周血CD3+T淋巴细胞百分比下降(P=0.003),CD8+T淋巴细胞百分比下降(P=0.002),外周血中IL-5浓度显著升高(P<0.01),小鼠骨髓有核细胞浓度及骨髓DNA含量降低(P=0.04,P<0.01)。牡蛎肽灌胃组小鼠的胸腺和脾脏显微结构恢复正常,高剂量牡蛎肽灌胃组小鼠的外周血白细胞数目与模型组相比升高(P=0.003),中剂量(P=0.04)和高剂量(P=0.02)的牡蛎肽灌胃组小鼠外周血CD3+T淋巴细胞百分比与模型组相比升高,高剂量的牡蛎肽灌胃组小鼠外周血CD8+T淋巴细胞百分比与模型组相比升高(P=0.002),各剂量牡蛎肽灌胃组的小鼠外周血中IL-5浓度与模型组小鼠相比显著降低(P值均<0.01),高剂量牡蛎肽灌胃组的小鼠外周血中IL-17浓度与对照组相比明显降低(P=0.03),各剂量牡蛎肽灌胃组的小鼠骨髓有核细胞浓度与模型组相比均明显升高(低剂量组vs.模型组,P=0.04;中剂量组vs.模型组,P=0.02;高剂量组vs.模型组,P=0.01),小鼠骨髓DNA含量与模型组相比也均明显升高(P值均<0.01)。结论:牡蛎肽可改善CTX所致免疫低下小鼠紊乱的免疫器官结构,恢复CTX造成的小鼠T淋巴细胞比例失调及细胞因子紊乱,升高骨髓有核细胞数及骨髓DNA含量,从而增强机体免疫功能。Objective： To evaluate the immunomodulating effect of oyster peptide on immunosuppressed mice. Methods： ICR mice injected with cyclophosphamide（ CTX） were adopted as the module group,with mice without treatment as the control group,and different dosages of oyster peptide（ 0. 5 g /kg,1. 0 g / kg,and 2. 0 g / kg） were given to the low,middle,and high groups for 15 days. The body weight,spleen,and thymus weight of the mice,structures under the microscope of the immune organs,numbers of white blood cells,ratios of T lymphocyte subsets,immune cytokines and numbers of nuclear cells,and DNA content in bone marrow were all assessed. Results： Compared with the control group,the structures of thymus and spleen of the mice in the CTX group appeared obscure and shrunk when observed under microscope,the number of their white blood cells declined（ P = 0. 04）,the proportion of their CD3＋T cells in peripheral blood declined（ P = 0. 003）,the proportion of their CD8＋T cells in peripheral blood declined（ P = 0. 002）,the concentration of their IL-5 in peripheral blood significantly increased（ P〈0. 01）,the concentration of their nucleated cells and DNA density in bone marrow decreased（ P = 0. 04,P〈0. 01）. Oyster could improve the structures of thymus and spleen of the immunosuppressed mice. Compared with the CTX group,the number of white blood cells in 2. 0 g / kg group increased（ P = 0. 003）,the proportion of CD3＋T cells in peripheral blood in 1. 0 g / kg group（ P = 0. 04）and 2. 0 g / kg group（ P = 0. 02） increased,the proportion of CD8＋T cells in peripheral blood in 2. 0 g /kg group increased（ P = 0. 002）,the concentration of IL-5 in peripheral blood in all the oyster treated groups increased（ P〈0. 01 in 0. 5 g / kg,1. 0 g / kg,and 2. 0 g / kg groups）,the concentration of IL-17 in peripheral blood in 2. 0 g / kg group decreased（ P = 0. 03）,the concentration of nucleated cells in bone marrow of all the oyster treated groups increased（ 0. 5 g / kg vs. CTX,P = 0

关 键 词：肽类 牡蛎科 环磷酰胺 免疫调节 生物活性肽 

分 类 号：R151.3[医药卫生—营养与食品卫生学]