光合细菌类胡萝卜素的提取工艺优化及抗氧化活性研究  

Study on optimization of carotenoids extraction in photosynthetic bacterium and their antioxidant activities

作　　者：李尽哲[1] 王伟[1] 黄雅琴[1,2] 

机构地区：[1]信阳农林学院生物与制药工程学院,信阳464000 [2]郑州大学河南省离子束生物工程重点试验室,郑州450052

出　　处：《中国食品添加剂》2016年第6期80-86,共7页China Food Additives

基　　金：河南省科技发展计划项目资助(152102210222)

摘　　要：为了建立一套相对简单、操作性强、提取率高的光合细菌类胡萝卜素提取工艺,利用单因素和正交试验优化了光合细菌类胡萝卜素的提取工艺。通过超声波辅助提取,分别考察了不同有机溶剂、超声时间、浸提时间、料液比4个因素对光合细菌类胡萝卜素的提取效果,最终获得的最佳提取条件为丙酮石油醚混合液(体积比1∶1)为提取溶剂,超声时间30 min,浸提时间4.5 h,料液比是0.1(g)/6.5(mL)。在该条件下提取,类胡萝卜素的提取量达到9432.56μg/g。通过不同浓度的类胡萝卜素及不同抗氧化剂对猪油POV值的影响试验,证明光合细菌类胡萝卜素具有较强的抗氧化活性,是一种来源于光合细菌、抗氧化效果较好的天然色素类食品添加剂。In order to establish an easier and high yield of extracting photosynthetic bacteria carotenoids,the extraction process of carotenoids in photosynthetic bacteria was optimized by single-factor test and orthogonal test. By using ultrasound-assisted extraction method,the effects of different ratios of organic solvents,ultrasonic times,extraction times and material to liquid ratios were tested in order to optimize extraction parameters. The optimum extraction conditions were：the extraction solution ratio of acetone and petroleum ether 1∶1,ultrasonic time 30 min;extraction time 3h,and the material liquid ratio 0.1g /6.5mL. The extraction carotenoid was 9432.56 μg /g under this condition. In addition,antioxidant effect of carotenoids was studied by lard POV value test at different concentrations of carotenoids and antioxidants. The results showed that bacteria carotenoids was a strong antioxidant and a natural food pigment derived from photosynthetic bacteria.

关 键 词：光合细菌 类胡萝卜素 提取工艺 优化 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]