野生蝉花多糖抗肿瘤活性及其作用机制  

Antitumor Activity and Mechanism of Action of Wild Cordyceps sobolifera Polysaccharide

作　　者：谢飞[1] 李伟[1] 陈美珍[1] 余杰[1] 

机构地区：[1]汕头大学理学院,广东汕头515063

出　　处：《食品科学》2016年第13期209-213,共5页Food Science

基　　金：2013年汕头大学教改项目;2013年广东省高等学校教学质量与教学改革工程本科类立项建设项目;高水平大学建设项目

摘　　要：目的：研究野生蝉花多糖（wildCordycepssoboliferapolysaccharide,CSP）体内外抗肿瘤活性.方法：以3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐（3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazoliumbromide,MTT）法考察不同剂量蝉花多糖体外对HeLa细胞的抑制作用,并以移植性S180实体荷瘤小鼠为模型检测体内抗肿瘤活性,通过机体免疫系统各项生化指标的测定,初步探讨其抗肿瘤作用机制.结果：当蝉花多糖质量浓度为400～800μg/mL时,对体外培养的HeLa细胞具有抑制作用,并呈量效关系,当作用质量浓度为800μg/mL时,抑制率达到61%.体内实验表明,蝉花多糖高剂量组（200mg/（kg·d））、低剂量组（100mg/（kg·d））均有-定抗肿瘤活性.当多糖给药剂量为200mg/（kg·d）（以体质量计）时,对S180荷瘤小鼠抑瘤率达到42.9%;同时,荷瘤小鼠免疫器官指数显著增大（P〈0.01）,并激活淋巴细胞的增殖分化,对抗氧化应激系统具有较好的保护作用.结论：野生蝉花多糖在体内、外均有明显的抗肿瘤活性,其原因可能与增强免疫调节能力及抗氧化活性有关.Objective： The antitumor activity of polysaccharides from wild Cordyceps sobolifera （CSP） was investigated invitro and in vivo. Methods： 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide （MTT） assay was used todetermine the anti-proliferative effect of CSP at different doses on HeLa cells. S180 sarcoma mouse model was establishedand used to detect the antitumor activity and the effect on the immune system of CSP. Results： CSP at concentrations of400？800 μg/mL inhibited the growth of HeLa cells in vitro in a concentration-dependent manner, with a percentage inhibitionof 61% at 800 μg/mL. In vivo, CSP at both high （200 mg/（kg·d）） and low （100 mg/（kg·d）） doses had antitumor effects, with apercentage inhibition of 42.9% at 200 mg/（kg·d）, and immune organ indices of tumor-burdened mice significantly increased（P 〈 0.01）. CSP also activated the proliferation and differentiation of lymphocytes, and had an obvious protection on theantioxidant defense system in the body. Conclusion： CSP has obvious antitumor activity in vitro and in vivo, which may berelated to enhancing the immune system adjustment capacity and antioxidant activity.

关 键 词：野生蝉花多糖 抗肿瘤活性 免疫调节 抗氧化 

分 类 号：R931[医药卫生—生药学]