HPLC-MS法测定米酒中的甜蜜素含量  

Determination of Sodium Cyclamate Content in Rice Wine by HPLC-MS

作　　者：张倩勉 

机构地区：[1]广西-东盟食品药品安全检验检测中心,广西南宁530021

出　　处：《酿酒科技》2016年第7期116-118,121,共4页Liquor-Making Science & Technology

摘　　要：通过HPLC-MS方法,同时采用正负离子模式进行多反应检测（MRM）,对米酒中的甜蜜素进行测定。结果表明,该方法的标准曲线线性良好,相关系数r=0.9998,检出限（3S/N）为0.1μg,回收率在85.4%~97.8%之间,RD值为2.9%~4.5%。该方法快速、简便、灵敏、准确,适宜于米酒中甜蜜素的快速测定。Sodium cyclamate content in rice wine was measured by HPLC-MS coupled with the positive and negative ions modes of MRM. The results showed that a good linearity was obtained for the standard curve of the method, its correlation coefficient was high （r=0.9998）, its detection limit was 0.1μg（3S/N）, its recovery rate was between 85.4%-97.8%, and RD value was 2.9%-4.5%. This method was rapid, sim-ple, sensitive and accurate, and it was a suitable method for rapid determination of sodium cyclamate content in rice wine.

关 键 词：HPLC-MS 米酒 甜蜜素 含量 

分 类 号：TS262[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]