螺旋藻规模生产优良藻株的选育  

Selection and breeding of fine Spirulina strains of large- scale production

作　　者：李正娟[1] 常蓉[1] 石光波[1] 徐青艳[1] 赵肖荣 闫卉新[1] 李博生[1] 

机构地区：[1]北京林业大学生物科学与技术学院食品科学与工程系,林业食品加工与安全北京市重点实验室(北京林业大学),北京林业大学螺旋藻研究所,北京100083

出　　处：《食品工业科技》2016年第14期210-213,218,共5页Science and Technology of Food Industry

基　　金：林业公益性行业科研专项(201304605)

摘　　要：本研究选用钝顶螺旋藻为出发株,采用毛细吸管显微分离法分离单藻丝,并结合紫外-可见光谱扫描,选育出了2个高产优质的螺旋藻藻株sina032和sina029。sina032和sina029的生物量较出发株分别提高了44.8%和39.4%,其生物活性物质(叶绿素、藻蓝蛋白、类胡萝卜素、β-胡萝卜素(顺式)和蛋白质)含量和出发株基本相同,且藻丝形态良好(藻丝较长、螺旋数约为20个、螺距较大)、上浮性较好。研究结果表明,通过单藻丝分离的方法,可选育出生物量高、生物活性物质含量高的钝顶螺旋藻藻株,在工业化生产中有着重要的实际意义。This study selected Spirulina platensis as original strain,and two strains sina032 and sina029 with high yeild and quality were obtained by using the method of capillary suction micro separation to separate per algal filament and combining with UV- Vis spectral scan. Compared with the original strain,biomass of the two strains increased by 44.8% and 39.4% respectively.Content of their bioactivators（ chlorophyll,phycocyanin,carotenoids,cis-β- carotene and protein） were oughly the same as that of the original strain.They had better filament form（ longer filament,about 20 spiral,bigger pitch） and higher flotation activity.These results showed that the method of separation of per algal filament can be used to screen Spirulina strains with high biomass and high content of bioactivators,which had important and practical significance in the industrial production of S.platensis.

关 键 词：钝顶螺旋藻 毛细吸管显微分离法 单藻丝 生物量 生物活性物质 

分 类 号：TS201.1[轻工技术与工程—食品科学]