超声波萃取黄秋葵籽油的脂肪酸组成及其抗氧化能力评价  

Fatty Acid Composition and Evaluation on Antioxidation Activities of Okra Seed Oil Under Ultrasonic Wave Extraction

作　　者：魏聪[1,2] 杨晓君[2] 王东晖[1] 方芳[1] 来吉祥[1] 王凤忠[1] 吴韬[1,3] 

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]新疆农业大学,乌鲁木齐830052 [3]西华大学食品与生物工程学院,成都610039

出　　处：《中国粮油学报》2016年第7期89-93,共5页Journal of the Chinese Cereals and Oils Association

基　　金：中国农业科学院基本科研业务增量项目(2014ZL042)

摘　　要：以黄秋葵种子为原料,正己烷为提取溶剂,采用超声波提取法提取黄秋葵种子中的籽油,气相色谱分析其提取物脂肪酸的种类和相对含量,并通过ABTS^+·自由基清除试验、DPPH自由基清除试验评价籽油的体外抗氧化能力,碘量滴定法测定过氧化值。结果表明,黄秋葵籽油中主要含有13种脂肪酸,以亚油酸(42.05%)、棕榈酸(26.43%)、油酸(20.58%)、十一烷酸(5.1%)、硬脂酸(3.4%,)为主,其过氧化值为5.86mmol/kg。体外抗氧化研究表明,黄秋葵籽油对DPPH自由基清除能力当量于0.5 mgVC/mL的清除能力,对ABTS^+·清除能力当量于285 mg Trolox/mL的清除能力。The okra seeds oil is obtained by the ultrasonic extraction method from okra seeds with hexane as the extraction solvent. The composition and relative content are also analyzed by the gas chromatography. The antioxidant capacity in vitro is evaluated by the ABTS＋ and DPPH radical scavenging experiments. In addition, the peroxide value of the sample is determined by the iodometric titration. The results show that okra seed oil mainly contains 13 kinds of fatty acids including linoleic acid （42.05%）, palmitic acid （26.43%）, oleic （20.58%）, undecanoate （5.1% ）, stearic acid （3.4%） and the peroxide value is 5.86 mmol/kg. The results of antioxidant activity in vitro indicate that the DPPH radical scavenging capacity of okra seeds oil is equal to 0.5 mg VC/mL, while the ABTS ＋ scavenging capacity is equal to 285 mg Trolox/mL.

关 键 词：黄秋葵籽油 脂肪酸 气相色谱 体外抗氧化 

分 类 号：TS224[轻工技术与工程—粮食、油脂及植物蛋白工程]