不同发酵方式对海南粗榧内生真菌CH1307c合成次级代谢产物的影响  

Effects of Different Fermentation Methods on Secondary Metabolites Synthesis by Endophytical Fungi CH1307c from Cephalotaxus hainanensis Li

作　　者：林淑婷[1] 李从发[1] 胡晓苹[1] 刘翊昊 徐传标 李培[1] 周偏 刘四新[1,2] 

机构地区：[1]海南大学食品学院,海南海口570228 [2]海南大学材料与化工学院,海南海口570228

出　　处：《热带作物学报》2016年第7期1407-1412,共6页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.313034);中国博士后科学基金(No.90279)

摘　　要：为研究不同发酵方式对内生真菌次级代谢产物产量、成分类别及其对3种常见白血病细胞株的细胞毒活性影响。分别采用大米固态发酵、麦麸固态发酵、马铃薯液态静置发酵、马铃薯动态发酵4种发酵方式对分离自海南粗榧韧皮部内生真菌CH1307c进行发酵,并采用乙酸乙酯进行萃取,通过试管定性试验和MTT试验测定其粗提物的成分类别及其对细胞株K562、NB4、HL60的细胞毒活性。结果表明:大米固态发酵、麦麸固态发酵的次级代谢粗提物产量相对于马铃薯动态发酵分别增加了45.9倍和28倍;马铃薯动态发酵较其静置发酵产量高9.1倍。试管定性试验结果表明,大米固态发酵获得的粗提物,化合物种类最多,麦麸发酵和动态发酵次之,马铃薯液态静置发酵最少。此外,4种发酵方式获得的代谢粗提物对3株细胞株的抗肿瘤活性(IC50值)并未呈现明显差异。研究结果为该菌株的大规模发酵提供理论依据。The effect of the different fermentation methods for the endophytic fungus on the yield of secondary metabolites component categories and the cytotoxic activities against the three common leukemia cell lines was investigated. The methods of rice solid-state fermentation, wheat bran solid-state fermentation, potato liquid-standing and potato dynamic fermentation were carried out for CH1307 c, which was isolated from phloem of Cephalotaxus hainanensis Li, and culture media were extracted with ethyl acetate. The categories of the crude extractions and their cytotoxic activities against cells K562, NB4 and HL60 were determined by using in vitro qualitative test and MTT test. Compared to the potato dynamic fermentation, the yields of secondary metabolites by rice and bran solid-state fermentation were increased by 45.9 and 28 times, respectively. In addition, the yield of potato dynamic fermentation was higher than its static way with 9.1 times. The results of in vitro qualitative test showed that component species of crude extraction by rice solid-state fermentation was richest, those by wheat bran solid-state and potato dynamic fermentation were second, and that by potato standing fermentation was fewest. Furthermore, the antitumor activities of secondary metabolites of the crude extractions by four fermentation ways towards the three cell lines（concentration of IC50value） did not exhibit obvious difference. This study provide a theoretical basis to the large-scale fermentation of the strain.

关 键 词：海南粗榧 发酵方式 内生真菌 化学成分 抗癌活性 

分 类 号：R284[医药卫生—中药学]