汾酒大曲中高产四甲基吡嗪菌株的筛选与鉴定  

Screening and identification of strains for tetramethyl pyrazine producing from Daqu for light fragrant Chinese-liquor making

作　　者：李晓霞[1] 李晶晶[1] 张秀红[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《食品与机械》2016年第6期49-51,共3页Food and Machinery

基　　金：山西省自然科学基金(编号:2012011034-5);山西省高等学校科技创新项目(编号:2013122);国家级大学生创新项目(编号:201410118003);山西省大学生创新项目(编号:2014153);山西师范大学创新项目(编号:SD2014CXXM-02)

摘　　要：从汾酒大曲中分离出15株可以产生TTMP的芽孢杆菌。通过观察菌落形态的基本特征和细胞的显微形态图对其进行纯种确认,采用气相色谱法筛选出3株高产TTMP的芽孢杆菌,并利用16SrDNA序列测定法进行菌种鉴定,得出3株高产TTMP的芽孢杆菌中2株为枯草芽孢杆菌、1株为弯曲芽孢杆菌,该结果增加了TTMP高产菌株的类型范围,为TTMP的生产实践应用提供了依据。Total 15 strains of Bacillus were isolated from Daqu for light fragrant Chinese liquor brewing.Their micro-morphology and clone characterize were observed.Three strains with higher TTMP yield were selected by gas chromatorgraphy,and identified as two Bacillus subtilis and one Bacillus flexus,and numbered as B76,B83 and B64,respectively,by molecular method.It broadens the strains for TTMP producing,laid a foundation for TTMP practical application in the future.

关 键 词：汾酒 四甲基吡嗪 气相色谱 16SrDNA 筛选 

分 类 号：TS262.3[轻工技术与工程—发酵工程]