正交试验优化超声辅助豆腐柴总黄酮提取工艺  

Optimizing the Ultrasound-assisted Extraction Techniques for Total Flavonoids from Premna microphylla Turcz by Orthogonal Test

作　　者：黄宏轶 黄玲艳[1] 张海燕[1] 李京雨[1] 孙翌[1] 彭景[1] 

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225127

出　　处：《安徽农业科学》2016年第18期132-133,224,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探索豆腐柴总黄酮的最佳提取工艺。[方法]在超声辅助和单因素试验基础上,采用三因素三水平正交试验优化得到豆腐柴总黄酮的最佳提取工艺。[结果]豆腐柴总黄酮最佳提取工艺为∶液料比为40∶1(m L/g),乙醇体积分数为70%,超声温度为70℃,提取时间为30 min。在此条件下,豆腐柴总黄酮提取率为83.1 mg/g。[结论]正交试验优化超声提取工艺能够显著提高豆腐柴总黄酮的提取率。Objective] To optimize the extraction techniques using for extracting total flavonoids from Premna microphylla Turcz.[Method] On the basis of ultrasound assisted and single factor test ,the optimal extraction technique was obtained through three factors and three levels orthogo-nal test.[ Result] The optimal extraction conditions were obtained as following：using 70% ethanol with a solid/liquid ratio of 40∶1 mL/g and extraction at 70℃for 30 min.Under the above conditions,83.1 mg/g flavonoids could be extracted from Premna microphylla.[Conclusion] The orthogonal test optimal ultrasound-assisted extraction technique can significantly improve the yield of total flavonoids from Premna microphylla.

关 键 词：豆腐柴 总黄酮 正交试验 工艺优化 

分 类 号：R284.2[医药卫生—中药学]