乳杆菌A2代谢产物LGF2对人口腔癌细胞增殖的抑制作用  

Inhibitory effect of Lactobacillus metabolites LGF2 on cell proliferation of oral cancer cells

作　　者：李美景[1] 关键[1] 关宏[2] 孙智辉 郭皓[2] 宋振宇 徐飞[1] 

机构地区：[1]黑龙江省佳木斯大学口腔医学院颌面外科,154004 [2]齐齐哈尔医学院微生态工程技术研究中心

出　　处：《齐齐哈尔医学院学报》2016年第15期1899-1901,共3页Journal of Qiqihar Medical University

基　　金：黑龙江省自然科学基金面上项目(编号:H201357)

摘　　要：目的考察乳杆菌A2(Lactobacillus A2)代谢产物LGF2对人口腔癌细胞CAL-27、KB细胞增殖的抑制作用。方法采用凝胶层析法对乳杆菌A2(Lactobacillus A2)的乳清蛋白代谢产物进行分离纯化,部分收集器收集,真空冷冻干燥法进行冷冻干燥。采用MTT法检测收集组分对人口腔癌细胞增殖的抑制作用。采用倒置光学显微镜观察经乳杆菌代谢产物作用后肿瘤细胞的形态变化。结果层析产物LGF2作用于人口腔癌细胞,倒置光学显微镜观察显示随着药物浓度的提高人口腔癌细胞数量逐渐减少;MTT法检测结果表现出随着药物浓度的提高癌细胞抑制率提高,LGF2对人口腔癌细胞系CAL-27、KB细胞增殖的抑制作用其对应的IC50分别为0.723 mg/m L和0.729 mg/m L。结论 LGF2对人口腔癌细胞系CAL-27、KB细胞增殖有抑制作用且呈剂量依赖性关系。Objective To investigate the inhibitory effect of LGF2 that the whey protein metabolites of A2（Lactobacillus sp.A2） on the proliferation of human oral tumor cells CAL-27 cells and KB cells.Methods The metabolites of whey protein associated A2（Lactobacillus sp.A2） was isolated and purified by ion-exchange method and gel chromatography method.Collected and dried the components with the vacuum freeze drying method.Then,the main active ingredients LGF2 were selected and the detection of morphologic variations by optical microscope and MTT method was used to detect the inhibitory effect on of inverted oral tumor cells.Results Inverted optical microscope observation showed that with the increase of the concentration of the drug the number of human oral tumor cells decreased gradually.MTT assay showed that the inhibition rate of CAL-27 cells and KB cells increased with the increase of drug concentration,its corresponding IC50 were 0.723 mg/ml and 0.729 mg/ml respectively.Conclusions LGF2 may inhibit the proliferation of CAL-27 cells and KB cells in a dose-dependent manner.

关 键 词：乳杆菌 乳清蛋白 分离纯化 MTT检测 

分 类 号：R737.33[医药卫生—肿瘤]