霍山石斛多酚超声波辅助提取工艺优化及其抗氧化活性研究  

Optimization on ultrasonic-assisted extraction of polyphenols from Dendrobium huoshanense and its antioxidant activity

作　　者：魏明[1] 刘艳艳[1] 蔡为荣[1] 钱森和[1] 张珂[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《食品与机械》2016年第7期136-140,共5页Food and Machinery

基　　金：安徽省自然科学基金(编号:1408085MC65);安徽省科技攻关项目(编号:1301032136)

摘　　要：为确定超声波辅助纤维素酶法提取霍山石斛多酚的最佳工艺,采用响应曲面设计方法对多酚提取工艺进行优化,同时分析霍山石斛多酚的抗氧化活性。结果表明:多酚提取的最佳工艺条件为超声功率180 W,超声时间20 min,酶质量浓度2.1 mg/m L,酶解温度57℃,酶解时间71 min,酶解p H 5,在该条件下,多酚平均得率为13.74 mg/g。体外抗氧化活性试验表明,霍山石斛多酚具有较强的抗氧化活性,其DPPH和ABTS自由基清除能力与多酚浓度呈现明显的量效关系。多酚对DPPH和ABTS自由基的半抑制浓度分别为0.057 mg/m L和0.027 mg/m L。In order to determine the optimal conditions for the extraction of polyphenols from Dendrobium huoshanense by ultrasonic-assisted cellulase,response surface methodology was used to optimize the extraction process. Antioxidant activity of Dendrobium huoshanense polyphenols was evaluated. The results showed that the optimal conditions for the extraction of polyphenols were as follows： ultrasonic power 180 W,ultrasonic time 20 min,cellulase concentra-tion 2. 1 mg / m L,enzymolysis temperature 57 ℃,enzymolysis time 71 min,and enzymolysis p H 5. Under these conditions,the average yield of polyphenols was 13. 74 mg / g. Antioxidant assays revealed that Dendrobium huoshanense polyphenols had significant antioxidant activity. DPPH and ABTS radical scavenging activities had a stronger relationship with the content of polyphenols,and their hemi-inhibitory concentrations（ IC50） were 0. 057 and 0. 027 mg/m L,respectively.

关 键 词：霍山石斛 多酚 超声波 提取 抗氧化 

分 类 号：TQ461[化学工程—制药化工]