荔枝果肉多糖级分的抗氧化和α-葡萄糖苷酶抑制活性研究  

Antioxidant Activity and α-Glucosidase Inhibitory Effect of Litchi Pulp Polysaccharide Fractions

作　　者：黄菲[1] 张瑞芬[1] 董丽红[1] 肖娟[1] 唐小俊[1] 张名位[1] 

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所/农业部功能食品重点实验室/广东省农产品加工重点实验室,广东广州510610

出　　处：《食品科学技术学报》2016年第4期26-30,49,共6页Journal of Food Science and Technology

基　　金：国家自然科学基金-广东联合基金重点项目(U1301211);广东省自然科学基金资助项目(2016A030310321);广东省科技计划项目(2016B070701012)

摘　　要：为了探究不同荔枝果肉多糖级分的理化性质、抗氧化能力和α-葡萄糖苷酶抑制活性差异,采用DEAE-52离子交换树脂分离纯化得到2种荔枝果肉多糖级分LPI和LPII,比较两者的中性糖、糖醛酸和蛋白质含量,并采用氧自由基清除能力(ORAC)和细胞抗氧化(CAA)评价其抗氧化活性,采用对-硝基酚-α-D吡喃葡萄糖苷(PNPG)法探究其α-葡萄糖苷酶抑制活性。研究结果表明LPII含有较多的中性糖和蛋白质,较少的糖醛酸,表现出更强的抗氧化和α-葡萄糖苷酶抑制活性,其中LPI和LPII的ORAC和CAA值分别是24.51,30.08μmol/g和5.36,8.72μmol/g,其抑制α-葡萄糖苷的IC_(50)值分别是0.33,0.26 mg/m L。荔枝果肉多糖的生物活性与其中性糖和蛋白质含量密切相关。In order to explore the differences of litchi pulp polysaccharide fractions,the content and composition,as well as antioxidant capacities and α-glucosidase inhibitory activities of varied litchi pulp polysaccharide fractions were studied. A DEAE-52 anion-exchange column was used to obtain two different litchi pulp polysaccharide fractions,denoted as LPI and LPII. The contents of neutral sugar,uronic acid,and protein were analyzed. In addition,their antioxidant activities were evaluated by oxygen radical absorbance capacity（ ORAC） and cellular antioxidant activity（ CAA）. The p-nitrophenyl-α-D-glucopyranoside method was used to measure the α-glucosidase inhibitory activity. The results showed that LPII contained more neutral sugar and protein than LPI,but less uronic acid. LPII exhibited higher antioxidant activity in ORAC value（ 30. 08 μmol/g） and CAA value（ 8. 72 μmol/g） that those of LPI（ 24. 51μmol/g and 5. 36 μmol/g）. In addition,the IC_（50） values of α-glucosidase inhibition were 0. 33 and 0. 26 mg/m L for LPI and LPII. The results demonstrated that bioactivities of litchi pulp polysaccharide fractions were related to its neutral sugar and protein contents.

关 键 词：荔枝 多糖 抗氧化 Α-葡萄糖苷酶 

分 类 号：TS255.2[轻工技术与工程—农产品加工及贮藏工程] TS201.2[轻工技术与工程—食品科学与工程]