利用甘蔗糖蜜发酵生产谷胱甘肽  

Producing of Glutathione(GSH) by Using Sugarcane Molasses

作　　者：何海燕[1,2] 卢福芝[1,2] 韦振菊 黄丽文[1,2] 覃拥灵 

机构地区：[1]河池学院化学与生物工程学院,宜州546300 [2]广西高校微生物及植物资源开发利用实验室,宜州546300

出　　处：《食品工业》2016年第8期85-87,共3页The Food Industry

基　　金：广西高等教育本科教学改革工程立项项目(2015JGA326);生物化工广西重点学科(河池学院)建设基金(201308)

摘　　要：以甘蔗糖蜜为发酵原料,经微波诱变选育得到的啤酒酵母SP3为发酵菌株发酵生产谷胱甘肽,通过单因素试验和正交试验设计,确定优化的培养条件为发酵液糖蜜糖度12°Bx,硫酸铵添加量2.5%,发酵液初始p H 5.5,28℃发酵96 h谷胱甘肽产量为75.327 mg/L,是未优化前产量61.06 mg/L的1.23倍。Glutathione（GSH） was produced by using sugar-cane molasses as fermentation material and Saccharomyces cerevisiae SP3 as fermenting strain,after the single factor and orthogonal design experiments,the optimal combinations were confirmed as follow：The content of sugarcane molasses was 12 °Bx,the ammonium sulphate content was 2.5%,initial p H of culture medium was 5.5,and then cultured on the shaking table at 28 ℃ for about 96 h,under the optimized conditions,the yield of glutathione was 75.327 mg/L,which was 123% higher than the initial fermentation conditions（61.06 mg/L）.

关 键 词：甘蔗糖蜜 谷胱甘肽 微波诱变 正交设计 

分 类 号：TS249[轻工技术与工程—制糖工程] TQ920.6[轻工技术与工程—食品科学与工程]