食品中17种反式脂肪酸的气相色谱测定方法  

Determination of trans fatty acids in food by gas chromatography

作　　者：陈月晓[1] 唐凌轩 李全霞[1] 崔亚娟[1] 何梅[1] 李东[1] 何涛 黄华 崔旸 路勇 

机构地区：[1]北京市营养源研究所,北京100069 [2]北京市食品安全监控和风险评估中心,北京100041

出　　处：《食品工业科技》2016年第17期292-296,共5页Science and Technology of Food Industry

基　　金：北京市科技计划课题首都居民营养膳食结构研究与数据平台搭建(Z141100002614013)

摘　　要：本文建立了适合食品中反式脂肪酸(Trans Fatty Acids,TFAs)的检测方法,并采集典型样品对其中的反式脂肪酸的含量进行测定,以了解其中的TFAs水平。加入内标物的试样(油脂试样除外)经水解-乙醚溶液提取其中的脂肪后,在碱性条件下皂化和甲酯化,生成脂肪酸甲酯,经毛细管气相色谱分析,内标法定量计算。结果表明,本研究条件下的反式脂肪酸甲酯能够有较好的分离,线性良好,线性相关系数R2均大于0.99。所采集的样品中有不同含量水平的反式脂肪酸,主要包括以下几类t C14∶1、t C15∶1、t C16∶1、t C18∶1、t C18∶2,根据样品类别的不同所含有的反式脂肪酸的类别也有所差异。A suitable method for trans fatty acids in food was established and the content of TFAs in typical samples was determined,in order to obtain the level of TFAs.Fat and fatty acdis in samples with internal standard were extracted by hydrolytic methods, then methylated to fatty acid methyl esters （FAMEs） using BF 3 in methanol. FAMEs were quantitatively measured by capillary gas chromatography（GC） against C11：0 internal standard.Under this condition TFAs had a good separation and linear,the linear co[relation coefficient R2 were greater than 0.99. Results showed the collection samples had different levels of trans fatty acids,which mainly include the following categories tC14：1 ,tC15： 1, tC16： 1, tCl8： 1, tC18： 2, according to the different types samples, the trans fatty acids category also differed.

关 键 词：食品 反式脂肪酸 气相色谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]