玉米多孔淀粉-海藻酸钠-壳聚糖-葡萄多酚缓释微胶囊的制备及表征  

Preparation and characterization of sustained release microcapsules of grape polyphenols with porous cornstarch, alginate sodium, and chitosan

作　　者：卢晓霆[1] 许中畅 王田田[1] 闫俊[2] 

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012 [2]吉林省轻工业设计研究院,吉林长春130012

出　　处：《中草药》2016年第13期2252-2259,共8页Chinese Traditional and Herbal Drugs

基　　金：吉林省科技发展计划项目(20120919);吉林省教育厅"十二五"科学技术研究项目(2014129)

摘　　要：目的为提高葡萄多酚的稳定性和强化缓释效果,采用玉米多孔淀粉为芯材载体复合凝聚法制备葡萄多酚微胶囊。方法以包埋率为主要指标,通过单因素试验和正交试验考察了各因素对葡萄多酚微胶囊化的影响,并对其制备工艺进行优化。结果优选的最佳工艺为25 mg/m L葡萄多酚溶液10 m L,玉米多孔淀粉用量1.5 g,在质量浓度0.03 g/m L海藻酸钠溶液30 m L、0.01 g/m L壳聚糖溶液50 m L、0.05 g/m L氯化钙溶液50 m L、p H 3.5时制得的微胶囊外观较优,粒径分布主要在600~850μm,葡萄多酚包埋率为83.2%,微胶囊产品在模拟胃液和肠液环境中具有很好的释放特性。结论以玉米多孔淀粉为芯材载体,海藻酸钠-壳聚糖为壁材制备的葡萄多酚微胶囊产品的形态及包埋率要优于单纯的海藻酸钠-壳聚糖微胶囊。用红外光谱和扫描电镜对葡萄多酚缓释微胶囊结构进行表征,均证实包埋物制备成功。Objective In order to improve the stability of grape polyphenols and strengthen slow-release effect, the study on microcapisulazed grape polyphenols was carried out through the complex coacervation method using porous cornstarch as core material carrier. Methods With the embedding rate as main index, the effect of all factors on the microencapsulation of grape polyphenols was investigated through the single factor test and orthogonal test, and its preparation technology was also optimized. Results The best preparation technology was as follows： The experiment materials were 10 m L grape polyphenols solution of 25 mg/m L, 1.5 g porous cornstarch, 30 m L sodium alginate solution of 0.03 g/m L, 50 m L chitosan solution of 0.01 g/m L, and 50 m L calcium chloride solution of 0.05 g/m L, at p H value of 3.5. The microcapsules＇ appearance was superior with size distribution of the main in 600—850 μm, the embedding rate was 83.2%, and they had very good releasing property in simulated gastric and simulated intestinal environment. Conclusion The product appearance and embedding rate of grape polyphenols microcapsules which used porous cornstarch as core material carrier and sodium alginate-chitosan as wall materials are better than those only used sodium alginate and chitosan as wall materials. Furthermore, the inclusion complex is proved to be successfully prepared by its structural characterization which is gotten from FTIR and scanning electron microscope（SEM）.

关 键 词：玉米多孔淀粉 海藻酸钠 壳聚糖 葡萄多酚 缓释微胶囊 包埋 稳定性 复合凝聚法 

分 类 号：R283.6[医药卫生—中药学]