不同温度对β-葡聚糖酶分批发酵动力学的影响  

Kinetic Study of β-Glucanase Production by Batch Fermentation at Different Temperatures

作　　者：赵希岳[1] 宋厚煜 蔡志强[1] 陈小花[1] ZHAO Xiyue SONG Houyu CAI Zhiqiang CHEN Xiaohua(School of Pharmaceutical and Life Science, Changzhou University, Changzhou 213164, China)

机构地区：[1]常州大学制药与生命科学学院,江苏常州213164

出　　处：《食品科学》2016年第19期211-215,共5页Food Science

基　　金：江苏省研究生培养创新工程项目(CX09S-010Z);江苏省企业研究生工作站项目(20130605)

摘　　要：在5 L发酵罐中研究不同温度对枯草芽孢杆菌（Bacillus subtilis）ZJF-15菌体生长、底物消耗和产酶量的影响.分批发酵的工艺条件为：搅拌转速400 r/min、通气量1.0 L/（L·min）、pH 7.0、接种量5%、装液系数0.6、发酵温度35～37 ℃.结果表明：温度升高会导致反应速率加大、生长代谢加快、生产期提前、酶失活速率加快、发酵周期缩短、最终产酶量减少.实验建立了B. subtilis ZJF-15分批发酵的菌体生长、底物消耗和β-葡聚糖酶产酶的动力学方程.The effect of different temperatures on the growth of Bacillus subtilis ZJF-15, substrate consumption andβ-glucanase production was studied by conducting batch fermentation experiments in a 5-L fermentation tank. Thefermentation conditions were determined as 400 r/min, 1.0 L/（L·min）, 7.0, 5%, 0.6 and 35–37 ℃ for rotating speed,ventilation rate, initial fermentation pH, inoculum quantity, medium loading coefficient and culture temperature, respectively.The results showed that increased culture temperature led to a higher reaction rate, accelerated bacterial growth andmetabolism, earlier production of β-glucanase, faster enzyme inactivation, shortened fermentation period and finally reducedproduction of β-glucanase. The kinetic equations for bacterial growth, substrate consumption and β-glucanase production ofBacillus subtilis ZJF-15 were established by batch fermentation.

关 键 词：枯草芽孢杆菌 Β-葡聚糖酶 分批发酵 温度 动力学 

分 类 号：Q815[生物学—生物工程]