69株食源性致病菌单核细胞增生李斯特氏菌的分子血清分型、二元基因分型与多位点序列分型研究  

Application of molecular serotyping,binary genotyping and multilocus sequence typing to subtype the 69 strains of food- borne listeria monocytogenes

作　　者：李秀娟[1] 高伟利[1] 史艳[1] 崔玲玲[2] 潘琢[1] 

机构地区：[1]石家庄市疾病预防控制中心,河北050011 [2]河北师范大学

出　　处：《医学动物防制》2016年第9期996-1000,共5页Journal of Medical Pest Control

基　　金：河北省医学科学研究重点课题(ZL20140244)

摘　　要：目的对多种分子分型方法在单核细胞增生李斯特氏菌(Listeria monocytogenes,Lm)分型中的应用进行分析和比较,确定适合于不同实验室的分型检测方法,并进一步掌握食源性Lm的病原特征。方法采用分子血清分型、二元基因分型与多位点序列分型方法对分离自食品的69株Lm进行分型检测,并对结果进行分析。结果 69株Lm食品分离株中,1/2a型菌株有29株,1/2b型有19株,1/2c型有7株,4b型有14株。二元基因分型法将69株Lm食品分离株分为19个二元基因型(Binary type,BT型),DI值为91.60%;主要的BT型为BT146、BT254、BT222、BT82、BT159和BT190。多位点序列分型法将69株Lm食品分离株分为16个序列型(Sequencing type,ST型),DI值为89.98%;主要的ST型为ST8、ST87、ST2、ST1、ST3和ST9。结论分子血清学方法和二元基因分型方法具有操作简便、检测结果易于分析、较快提供检测结果的优点,可作为实验室的一线技术用以对李斯特氏菌病暴发的发现和确认。Objective To screen the suitable type detection Methods in different laboratories by analyzed a variety of molecular typing methods in Listeria monocytogenes（ Lm）,and to further understand the characteristics of food borne Lm. Methods Molecular serotyping,binary genotyping and multilocus sequence typing method were used to subtype 69 Lm isolated,and the results were analyzed. Results The 69 isolates were typed into four serotypes,1 /2a,1 /2b,1 /2c and 4b. The most seroyptes were 1 /2a（ 29strains）,1 /2b（ 19 strains）,4b（ 14 strains） and 1 /2c（ 7 strains）. 69 isolates were divided into 19 binary types（ BTs）,index of diversity（ DI） was 91. 60%; the main type of BTs were BT146,BT254,BT222,BT82,BT159 and BT190. MLST analysis divided 69 isolates into 16 sequence types（ STs）,DI was 89. 98%,the main STs type were ST8,ST87,ST2,ST1,ST3 and ST9. Conclusion Molecular serotyping and binary genotyping method are simple,rapid and easy to analyze,they can be used as first- line laboratory methods to long- term monitoring for identification and sourcing of outbreaks of listeriosis.

关 键 词：食源性致病菌 单核细胞增生李斯特氏菌 分子血清分型 二元基因分型 多位点序列分型 溯源检测 

分 类 号：R378.99[医药卫生—病原生物学]