紫外线和硫酸二乙酯复合诱变选育PHB高产菌株  

Mutagenesis of poly-β-hydroxybutyricacid producing strain by UV and diethyl sulfate

作　　者：刘俊梅[1] 王庆[1] 王丹[2] 杨盼盼[1] 丁伟[1] 朴春红[1] 王玉华[1] 于寒松[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]吉林医药学院,吉林吉林132013

出　　处：《微生物学通报》2016年第10期2242-2248,共7页Microbiology China

基　　金：吉林省科技支撑重大攻关专项项目(No.20116034)~~

摘　　要：【目的】获得高产聚β-羟基丁酸酯(Poly-β-hydroxybutyricacid,PHB)菌株。【方法】以假单胞菌属Pseudomonas koreensis PK3菌株为出发菌株,采用硫酸二乙酯和紫外线相结合的诱变方法进行多轮诱变。【结果】经过初筛和复筛得到一株高产PHB突变菌株,命名为Pseudomonas koreensis UVCN-18。连续传代9次后,发酵28 h条件下,PHB产量达到15.94 g/L,占细胞干重69.54%,较原出发菌株Pseudomonas koreensis PK3(4.42 g/L)提高了2.61倍,并且该菌株具有良好的遗传稳定性。【结论】采用硫酸二乙酯和紫外线相结合的诱变方法,成功获得了一株高产PHB突变菌株。[Objective] We aim to get a high-yield poly-β-hydroxybutyricacid（PHB） mutant strain. [Methods] We use composite multiple rounds of mutagenesis breeding of UV and diethyl sulfate and after primary and secondary screening from the original strain Pseudomonas koreensis PK3. [Results] It＇s named Pseudomonas koreensis UVCN-18. After serially passed 9 times and fermentation 28 h, PHB production of the mutant was 15.94 g/L, Accounting for cell dry weight 69.54%. PHB production of the mutant was increased by 2.61 times compared with the original strain Pseudomonas koreensis PK3（4.42 g/L）, and the strain has a good genetic stability. [Conclusion] We use composite multiple rounds of mutagenesis breeding of UV and diethyl sulfate to get a high-yield PHB mutant strain.

关 键 词：紫外线 硫酸二乙酯 PHB 

分 类 号：Q93[生物学—微生物学]