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作 者:谢逸欣[1] 陈文秋[2] 曾婷[1] 李晓凤[3] 吴晖[3]
机构地区:[1]广州军区疾病预防控制中心,广东广州510507 [2]中山医科大学附属第六医院,广东广州510655 [3]华南理工大学轻工与食品学院,广东广州510640
出 处:《食品工业科技》2016年第20期134-137,144,共5页Science and Technology of Food Industry
摘 要:目的:研究不同提取工艺得到的不同玛咖提取物对精子功能的影响。方法:选取20名不孕男性精液,通过非连续密度梯度法制备精子悬液,将碱溶酸沉法、大孔吸附树脂吸附-解析法与水提法提取的玛咖中不同成分作用于精子悬液,通过计算机辅助精子分析系统(CASA)分析其对精子功能的影响。结果:代表精子活力参数的PR(前向运动精子)、PR-A(快速直线运动精子)和PR-B(中等前向运动精子),在玛咖水提物浓度为0.1 g/m L时,精子活力最佳,分别比对照组高54.67%(p<0.05)、52.74%(p<0.05)、58.15%(p<0.05);代表精子运动功能的参数在玛咖水提物浓度为0.1 g/m L时运动功能最佳,其中STR(直线速度与平均路径速度比值)、LIN(直线速度与曲线速度比值)、分别比对照组高1.61%(p<0.05)、13.32%(p<0.05)。结论:玛咖水提物在适宜的浓度下对精子的刺激作用使其活力达到高峰,能最大限度保持精子的高活动性及保证精子质量。Objective:To compare the effect of Maca extract in different process on human sperm function.Methods: Sperm suspension was prepared by the method of non- continuous density gradient in 20 infertile men, Maca ingredients were extracted by alkali soluble and acid sinking method, static and dynamic adsorption-desorption method, aqueous and hydroalcoholic extracting method.The effect of different Maca extract was analyzed on human sperm function by computer-aided sperm analysis system. Results: Values of PR (progressive), PR-A (progressive straight line)and PR-B (medium forward)in experimental group was separately higher 54.67%, 52.74% ,58.15% than control group(p 〈0.05).Sperm motility was the best when the Maca extract concertration was 0.1 g/mL,the value of STR ( straightness, VSL/VAP) and LIN (linearity) in experimental group was separately higher 1.61% and 13.32% than control group(p 〈 0.05 ).Conclusion: Experiment showed that sperm motility could reach the peak,sperm quality was beterwhen Maca extract was at a suitable concentration.
分 类 号:TS201.1[轻工技术与工程—食品科学]
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