机械搅拌发酵产细菌纤维素的中试研究  

Mechanical stirring fermentation pilot study of bacterial cellulose

作　　者：许云华[1] 朱春林[2] 张衡[2] 孙汴京 自强 顾焱[2] 孙东平[2] 

机构地区：[1]连云港师范高等专科学校生命科学系,江苏连云港222006 [2]南京理工大学化学生物功能材料研究所,江苏南京210094

出　　处：《食品工业科技》2016年第20期183-188,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(21206076);江苏省高等职业院校国内高级访问学者计划资助项目(2015FX032);江苏省高校自然科学研究面上项目资助(16KJB180034)

摘　　要：细菌纤维素的中试发酵实验研究是其工业化生产的必经阶段,有着极为重要的研究价值和意义。利用实验室20 L-50 L-100 L机械搅拌发酵罐系统对发酵生产细菌纤维素进行了中试实验,测定了葡糖杆菌在20 L种子罐中的生长曲线,研究了不同培养级数的种子对100 L机械发酵罐的影响,采用转速-溶氧（DO）联动控制法提高发酵中后期的溶氧水平和纤维素产量。结果表明,在种子生长曲线对数期的中后期（30-48 h）移种较佳,使用三级种子移种比一级和二级种子移种的发酵周期分别短62 h和14 h,保持移种后发酵罐的菌体浓度在2.0×10^7CFU/m L以上对缩短发酵过程延滞期有利,转速-DO联动控制可保证发酵中后期DO维持在30%左右,纤维素产量由2.2 g/L提高至2.8 g/L。细菌纤维素的中试实验结果对探索生产工艺、缩短发酵周期、降低生产成本、提高纤维素产量等方面有明显改善,为其工业化生产奠定了基础。The pilot fermentation experiment of bacterial cellulose （BC）is a necessary stage for its industrial production.It has a very important value and significance.The pilot fermentation experiment were systematically researched using 20 L- 50 L- 100 L laboratory stirred tank fermentation system. The growth curve of Komagataeibacter nataicola seeds at 20 L fermentor were determined.The effects of different seed culture series on 100 L mechanical fermentation tank were studied.To improve the level of dissolved oxygen, stirring speed- dissolved oxygen（DO） linkage control method were used during fermentation and cellulose production.The results showed that suitable inoculation period was in the middle and late stage of logarithmic phase （30-48 h）.The fermentation period using grade three seed was 62 h and 14 h shorter than grade one and two, respectively.To shorten the fermentation process delay time,the bacteria concentration in the fermentation tank was more than 2.0 × 10^7 CFU/mL after inoculation with bacteria.Stirring speed-DO linkage control could ensure DO maintained at around 30% and increased BC yield to 2.8 g/L.This research was beneficial with shortening the fermentation cycle, reducing the production cost, improving the yield of cellulose, and laying foundations for the industrial production. The pilot experiment study results of BC had a significant improvement in the production process.

关 键 词：细菌纤维素 中试研究 机械搅拌 发酵周期 

分 类 号：TS201.1[轻工技术与工程—食品科学]