外源添加代谢中间产物对米根霉生产富马酸的影响  

Effects of supplemented metabolic intermediates on production of fumaric acid by Rhizopus oryzae

作　　者：李鑫[1,2,3] 欧阳水平 陈晓佩[3] 周瑾[3] 

机构地区：[1]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037 [2]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037 [3]南京林业大学化学工程学院,江苏南京210037

出　　处：《生物加工过程》2016年第5期1-5,共5页Chinese Journal of Bioprocess Engineering

基　　金：江苏省高校自然科学研究重大项目(14KJA220003);江苏省自然科学基金(BK20131426);江苏省重点研发计划(BF2015007)

摘　　要：通过外源添加代谢中间产物(苹果酸、草酰乙酸和丙酮酸),研究其对米根霉积累富马酸及代谢关键酶的影响。结果表明,添加4.0 g/L苹果酸时,富马酸质量浓度可达34.0 g/L,与对照相比提高了24.5%,同时提高富马酸酶(FUMR)比酶活,抑制乙醇脱氢酶(ADH)比酶活。添加少于1.0 g/L草酰乙酸可提高FUMR比酶活,降低ADH比酶活,促进富马酸积累;但添加高于1.0 g/L草酰乙酸抑制了FUMR和ADH比酶活,导致富马酸和乙醇产量下降。添加1.5 g/L丙酮酸,使富马酸的质量浓度从27.3 g/L增加到38.7 g/L,提高了41.8%,但FUMR比酶活下降34.7%,而ADH比酶活保持稳定。Effects of supplemented metabolic intermediates on production of fumaric acid and key enzymes were studied for Rhizopus oryzae fumaric-malic acid producing strain. R. oryzae could produce 34. 0 g / L fumaric acid when 4. 0 g / L malic acid was added in the medium,which was 24. 5% higher than that of the control. Addition of malic acid increased specific activity of fumarase（ FUMR） and decreased specific activity of alcohol dehydrogenase（ ADH） in R. oryzae. Adding less than 1. 0 g / L oxaloacetate increased specific activity of FUMR and improved fumaric acid production by R. oryzae. However,adding more than1. 0 g / L oxaloacetate in the medium decreased both FUMR and ADH specific activities in R. oryzae,which resulted in a sharp decrease of fumaric acid and ethanol. The addition of 1. 5 g / L pyruvate improved fumaric acid production from 27. 3 g / L to 38. 7 g / L. Compared to the control,41. 8% increase of fumaric acid was observed. At the same time,a 34. 7% decrease of FUMR specific activity was observed with no change to ADH specific activity.

关 键 词：米根霉 代谢中间产物 富马酸 富马酸酶 乙醇脱氢酶 

分 类 号：TQ353.6[化学工程]