基于智能可视化优化上的米根霉发酵生产富马酸过程的优化  

Optimal process of producing fumaric acid by Rhizopus oryzae based on intelligent visualization

作　　者：彭程[1] 刘颖[1] 顾帅[1] 徐晴[1] 李霜[1] 黄和[2] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]南京工业大学药学院,江苏南京211800

出　　处：《生物加工过程》2016年第5期17-21,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2013CB733605);国家高技术研究发展计划(863计划)(2011AA02A206)

摘　　要：为提高米根霉发酵产富马酸的效率,对米根霉发酵过程进行了优化。通过单因素实验考察不同氮源对富马酸合成的影响,确定了米根霉ME-F14发酵产富马酸的最佳氮源为(NH4)2SO4;在此基础上采用均匀实验设计法进行试验设计,并利用智能可视化优化软件对发酵培养基的组分和培养条件进行优化。当接种龄为12 h、葡萄糖87.5 g/L、(NH4)2SO40.55 g/L、接种量27.5%时,富马酸产量达43.8 g/L,比对照组提高了31.81%。此结果可以为发酵法制备富马酸的工业化生产奠定基础。In order to enhance fumaric acid production by Rhizopus oryzae,we optimized the process of fermentation.（ NH4）2SO4was found to be the best nitrogen source for fumaric acid production by comparing different nitrogen sources. After that,a novel visualization method was developed to further optimize the fermentation process for fumaric acid production based on uniform experiment. Glucose concentration,（ NH4）2SO4concentration,inoculums concentration and inoculums time were designated as the four factors in uniform experiment. Results were handled and predicted by the intelligent visualized optimal software,fumaric acid production was considered as the objective. Consequently,verified test revealed that the optimized condition for fumaric acid production was with glucose concentration 87. 5g / L,（ NH4）2SO4concentration 0. 55 g / L,inoculums concentration 27. 5% and inoculums time at 12 h.Under this condition,fumaric production was 43. 8 g / L.Our findings provides a reference for the industrial production of fumaric acid by fermentation.

关 键 词：富马酸 米根霉 均匀设计 智能可视化优化 

分 类 号：TQ921[轻工技术与工程—发酵工程]