酸性木聚糖酶交联酶聚集体的制备条件优化  

Optimizing Preparation Conditions of Cross-linked Acid-stable Xylanase Aggregates

作　　者：吴春玲[1] 李璠[1] 马超[1] 袁月[1] 范光森[1,2,3] 李秀婷[1,2,3] 

机构地区：[1]北京工商大学食品学院,北京100048 [2]北京市食品风味化学重点实验室,北京100048 [3]北京食品营养与人类健康高精尖创新中心,北京100048

出　　处：《食品科学技术学报》2016年第5期48-54,共7页Journal of Food Science and Technology

基　　金：国家自然科学基金面上项目(31371723);北京市自然科学基金青年项目(6164029)

摘　　要：交联酶聚集体法是一种新型的无载体酶固定化方法,为提高酸性木聚糖酶的稳定性,使用该法固定化微紫青霉产酸性木聚糖酶,制备无载体固定化木聚糖酶,并对其制备条件进行优化。结果表明,优选的制备条件为将质量浓度0.36 mg/m L的酸性木聚糖酶粗酶液在冰水浴中经饱和质量分数为85%的硫酸铵沉淀30 min后,于40℃,加入终体积分数为0.14%的戊二醛,交联4 h可获得较高活性的交联酶聚集体,酶活保留率达42.2%。这有助于酸性木聚糖酶更好地在工业中应用。The cross-linked enzyme aggregates method was a new carrier-free immobilized enzyme tech- nology. In order to improve the stability of xylanase, the technology was applied for the preparation of the acid-stable xylanase from Penicillium janthinellum. This study explored conditions of the preparation of cross-linked xylanase aggregates. The results showed that the crude xylanase at the concentration of 0.36 mg/mL was precipitated with 85% saturated ammonium sulfate for 30 min at 40 ℃. Then the aggregates were cross-linked by 0.14% glutaraldehyde solution for 4 h at 40℃. The obtained activity of cross- linked xylanase aggregates remained 42.2% of total enzyme activity, which is helpful for the further ap- plication of acid xylanase in the industry.

关 键 词：微紫青霉 酸性木聚糖酶 交联酶聚集体 无载体固定化 

分 类 号：TS201.1[轻工技术与工程—食品科学] Q814.2[轻工技术与工程—食品科学与工程]