朝鲜毛球蚧各龄期虫体的挥发物成分分析  

Analysis on volatile components of Didesmococcus koreanus Borchsenius in different instars

作　　者：李伟[1] 张艳峰[1] 谢映平[1] 牛秀萍[1] 薛皎亮[1] 

机构地区：[1]山西大学生命科学学院,太原030006

出　　处：《环境昆虫学报》2016年第5期1003-1010,共8页Journal of Environmental Entomology

基　　金：国家自然科学基金-青年科学基金项目(31200495);山西省青年科技基金(2011021029-2)

摘　　要：为明确朝鲜毛球蚧Didesmococcus koreanus Borchsenius自身挥发物中所包含的信息素组分,采用顶空固相微萃取和气质联用(HS-SPME-GC/MS)的方法对该蚧虫各龄期虫体的挥发性气味成分进行研究。分析得出100μm PDMS萃取纤维对虫体的挥发性成分吸附性能较好,再以其吸附2龄若虫、3龄若虫、雌成虫以及虫卵的挥发性成分,并比较了不同龄期、不同虫态挥发性成分组成的异同和数量差异。结果表明,朝鲜毛球蚧的挥发性成分主要有烷烃类、醇类、酮类、酯类、含苯环化合物等29种成分。随着龄期的增加,虫体挥发物的种类由2龄若虫的22种,3龄若虫的15种,逐渐减少到雌成虫的10种,且不同发育阶段的挥发性组分有明显差异。在若虫期1-辛烯-3-醇的相对含量都是最大的,分别占25.28%和22.51%;发育到雌成虫阶段,2-十一酮增加到21.85%为相对含量最大的组分;而虫卵中邻二甲苯(48.71%)相对含量最大。通过分析挥发物的变化规律,结合其他研究结果,初步推测1-辛烯-3-醇是朝鲜毛球蚧的一种重要的信息素成分,2-壬酮、2-十一酮很有可能是朝鲜毛球蚧的性信息素,通过此次研究为朝鲜毛球蚧的天敌引诱剂和性引诱剂的制备提供科学依据。In order to investigate the pheromone component contained in the body volatiles of Didesmococcus koreanus Borchsenius,these compounds in different instars were studied by headspace solid phase micro-extraction and gas chromatography-mass spectrometry（HS-SPME-GC/MS）. The fiber of 100μm PDMS had the best adsorption property of body volatile compounds by analysis. The similarities and differences of composition and quantity differences in different instars were compared after adsorbing of volatile compounds of 2ndinstar nymph,3rdinstar nymph,female adult and egg by this fiber. The results showed that a total of 29 compounds were analyzed and identified,including alkanes,alcohols,ketones,esters,and benzene compounds and so on. The types of body volatile compounds gradually reduced with the growth（2ndinstar nymph containing 22 kinds,3rdinstar nymph containing 15 kinds, female adult containing 10 kinds） and there was significant difference on volatile component of D. koreanus in different developmental stages. The relative content of 1-Octen-3-ol was the highest in the 2ndinstar nymph（25. 28%） and 3rdinstar nymph（22. 51%）; And 2-Undecanone reached 21. 85% which was the highest one in the adult stage; While the o-Xylene was the highest that accounted for 48. 71% of all eggs volatiles.1-Octen-3-ol was preliminarily speculated to be a important pheromone component of D. koreanus,2-Nonanone and 2-Undecanone were likely to be the sex hormone of D. koreanus through the analysis of the change rule of volatile matter and the comparison with other research results. The research provided scientific data for the preparation of attractants for natural enemies and sexual attractants of D. koreanus.

关 键 词：朝鲜毛球蚧 固相微萃取 气质联用 信息素 

分 类 号：Q966[生物学—昆虫学] S433.39[农业科学—农业昆虫与害虫防治]