免疫亲和层析净化高效液相色谱法测定澳洲坚果中黄曲霉毒素B_1  

Determination of Flatoxin B_1 in Macadamia by Immunoaffinity Column and High Performance Liquid Chromatography

作　　者：林玲[1] 李琪[1] 刘丽丽[1] 张利强[1] 杨春亮[1] 

机构地区：[1]中国热带农业科学院农产品加工研究所农业部农产品加工质量安全风险评估实验室(湛江),广东湛江524001

出　　处：《化学试剂》2016年第11期1086-1088,1091,共4页Chemical Reagents

基　　金：2016年农产品产地初加工危害因子识别与关键点控制评估专项项目(GJFP201601102);2016年国家柑橘及热带果品质量安全风险评估项目(GJFP2016004);海南省自然科学基金资助项目(0110200027)

摘　　要：建立了一种测定澳洲坚果中黄曲霉毒素B1的免疫亲和层析净化高效液相色谱法。采用甲醇-水提取,提取液经过滤、水稀释、净化,甲醇洗脱溶解,柱后衍生液相色谱仪荧光检测器测定。实验结果表明,澳洲坚果中黄曲霉毒素B1的检出限为0.2μg/kg,在添加水平为5、10、20μg/kg的回收率实验中,平均回收率为81%～96%,相对标准偏差为0.8%～3.7%。A method for the determination of aflatoxin B1 in macadamia was established by immunoaffinity column and high performance liquid chromatography. The samples extracted with methanol-water were filtered,diluted with water and through to immunoaffinity column for cleaning. Then the residues was dissolved using methanol and determination by post column derivatization HPLC fluorescence detection. The results showed that the detection limits of aflatoxin B1 reached 0. 2 μg / kg. The recovery were obtained by three different concentration levels（ 5,10 and 20 μg / kg） to the blank sample. The mean recovery rates were between81%～96%,and the relative standard deviation were 0. 8%～ 3. 7%.

关 键 词：免疫亲和层析 高效液相色谱 黄曲霉毒素B1 澳洲坚果 柱后衍生 

分 类 号：R15[医药卫生—营养与食品卫生学]