响应面法优化亚临界水提取槟榔籽中槟榔碱的工艺研究  

Optimization of subcritical water extraction process of arecoline from areca seeds by response surface methodology

作　　者：康丽如[1] 符素芳 曾广琳[1] 姬彦羽 张海德[1] 张伟敏[1] 

机构地区：[1]海南大学食品学院,海口570228

出　　处：《食品安全质量检测学报》2016年第9期3773-3780,共8页Journal of Food Safety and Quality

基　　金：天津大学-海南大学协同创新基金项目~~

摘　　要：目的利用响应面试验优化亚临界水提取槟榔籽中槟榔碱的工艺。方法在单因素实验的基础上,利用单因素试验结合Box-Benhnken实验和响应面分析法,研究提取温度、提取时间以及液料比3个因子对槟榔籽槟榔碱提取率的影响,通过回归分析模拟得到二次多项式回归方程的预测模型。结果亚临界水提取槟榔籽中槟榔碱的最佳工艺条件为提取温度144℃,提取时间43 min,液料比35:1(mL/g)。由模型预测提取率为4.23mg/g,验证实验结果为4.25 mg/g,误差仅为0.47%,与预测值较接近,表明模型具有较好预测性。且各因子对提取率的影响大小依次是提取温度>提取时间>液料比。结论亚临界水提取可以作为一种有效的槟榔籽中槟榔碱提取方法。Objective To investigate the optimization of subcritical water extraction process of arecoline from areca seed（AAS） by response surface methodology.Methods The single-factor tests and response surface methodology were used to investigate the effect of three parameters（extraction temperature,extraction time and liquid-solid ratio） on the yield of AAS.A second- order polynomial regression model was established using a Box-Behnken experimental design.Results The optimum process conditions were as follows：extraction time of 43 min,extraction temperature of 143℃,liquid-to-solid ratio of 35：l（mL/g）,and the extraction rate of AAS was up to4.25 mg/g,which was highly consistent with the predictive value of 4.23 mg/g,with the fractional error of 0.47%.It showed that the established regression equation for arecoline yield exhibited an excellent goodness of fit,and the orders of factors influencing the extraction of AAS were as follows：extraction temperature〉extraction time〉liquid-to-solid ratio.Conclusion Subcritical water extraction can be applied for the extraction of AAS.

关 键 词：槟榔籽 槟榔碱 亚临界水提取 响应面 

分 类 号：TQ914.1[化学工程]