液态发酵生产水溶性红曲黄色素的条件优化  

Optimization of liquid-state fermentation conditions for the production of water soluble yellow Monascus pigment

作　　者：李瑞杰[1] 谢玉梅[1] 何思琴[1] 王磊[1] 陈莎[1] 高梦祥[1] 李利[1] LI Ruijie XIE Yumei HE Siqin WANG Lei CHEN Suo GAO Mengxiang LI Li(College of Life Science, Yangtze University, Jingzhou 430025, Chin)

机构地区：[1]长江大学生命科学学院,湖北荆州430025

出　　处：《中国酿造》2016年第11期73-77,共5页China Brewing

基　　金：国家自然科学基金资助项目(31501453;31371829);长江大学大学生创新创业训练计划项目(20150089)

摘　　要：以红色红曲菌（Moaascus ruber）M-7为实验菌株，以色价和色调为评价指标，对液态发酵生产水溶性红色红曲黄色素的条件进行了研究。结果表明，培养基pH值为3～4时，红色红曲菌M．7能合成大量的水溶性红曲黄色素，发酵液呈鲜艳的黄色色调；培养基最优碳源为2％蔗糖，最优氮源为0．2％硫酸铵；水溶性红色红曲黄色素的色价和色调值随着培养时间的延长先升后降，在第8天时达到最高，色价为5．56U／mL，OD400nm/OD510nm为10．29，OD400nm／OD470nm为3．93。Using Monascus tuber M-7 as experimental strain, color value and color tone as evaluation index, the production of water soluble yellow Monascus pigment by liquid state fermentation was investigated. The results showed that when the fermentation medium pH was 3-4, a large portion of water soluble yellow Monascus pigment was synthesized by M. tuber M-7, and the fermentation broths was bright yellow. The optimal carbon source of the medium was sucrose 2%, and the optimal nitrogen source was ammonium sulfate 0.2%, respectively. The color value and the color tone of water soluble yellow Monascus pigment increased in the early stage, decreased in the late stage, and the maximum points were on the 8th day. After 8 d cultivation, the production of water soluble yellow Monascus pigment was 5.56 U/ml,OD400nm/OD510nm was 10.29 and OD400nm/OD470nm was 3.93.

关 键 词：红色红曲菌 水溶性红曲黄色素 液态发酵 

分 类 号：TS264.4[轻工技术与工程—发酵工程]