绿色木霉和黄孢原毛平革菌混合降解稻草秸秆中的VOCs  

Volatile productions during mixed culture to degrade straw between Trichoderma viride and Phanerochaete chrysosporium

作　　者：陈耀宁[1,2] 谭雪斌 黎媛萍[3] 马騻 张洁[1,2] 苟宇[1,2] 刘耀[1,2] 汪元南 朱福造 张道利[1,2] 陈艳容[1,2] CHEN Yaoning TAN Xuebin LI Yuanping MA Shuang ZHANG Jie GOU Yu LIU Yao WANG Yuannan ZHU Fuzao ZHANG Daoli CHEN Yanrong(College of Environment Science and Engineering, Hunan University, Changsha 410082, China Key Laboratory of Environmental Biology and Pollution Control （Hunan University） , Changsha 410082, China College of Municipal and Mapping Engineering, Hunan City University, Yiyang 413000, China)

机构地区：[1]湖南大学环境科学与工程学院,长沙410082 [2]环境生物与控制教育部重点实验室(湖南大学),长沙410082 [3]湖南城市学院市政与测绘工程学院,益阳413000

出　　处：《环境工程学报》2016年第11期6635-6641,共7页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(51378190;21276069;51579094);湖南省自然科学基金项目(13JJB002);中央高校基本科研业务费专项

摘　　要：利用黄孢原毛平革菌和绿色木霉混合培养降解稻草秸秆,使用顶空固相微萃取气质联用的方法研究了降解过程中产生的挥发性有机物,并对混合培养中同一平板上不同区域产生的挥发性有机物进行了比较。纯培养和混合培养中挥发性有机物的对比研究表明,两株菌的混合培养较其纯培养有更强地降解稻草的能力。在混合培养过程中检测到C15H24的两种同分异构体,分别属于双环倍半水芹烯和环己二烯类物质,均是倍半萜烯,证实了黄孢原毛平革菌和绿色木霉混合培养时的拮抗机制。同时发现环己二烯类物质仅在混合培养中的混合区域检测到,表明混合培养中混合区域较之非混合区域具有更强的竞争表现,分区域检测降解过程中挥发性有机物能更好地研究混合接种的降解机理。Volatile organic compounds（ VOCs） in mixed and pure cultures of Trichoderma viride and Phanerochaete chrysosporium were quickly detected by headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry（ HS-SPME-GC-MS）,and the VOCs of different areas in the same Petri dish were identified. The VOCs detected in mixed and pure cultures showed that to some extent,the mixed culture could degrade the straw more efficiently. Two sesquiterpenes（ bicyclosesquiphellandrene and cyclohexadiene） were identified only in the mixed culture,indicating an antagonistic relationship between T. viride and P. chrosospoorium. The sesquiterpene cyclohexadiene was detected only in the interaction area of the mixed culture,suggesting that the lignocellulose degradation mechanism in the interaction area of the mixed culture is most likely different from that in other areas. The comparison of VOCs of different areas in the same Petri dish could be considered as an effective way to analyze the lignocellulose degradation mechanism in mixed cultures.

关 键 词：顶空固相微萃取 气质联用 混合接种 挥发性有机物 生物降解 

分 类 号：X172[环境科学与工程—环境科学]